官方网址：http://www.sinobiological.cn/Granzyme-A-GZMA-cDNA-Clone-g-922.html
 General Information 
 
Manufactured By:  Sino Biological Inc. 
 
 Gene Bank Ref.ID:   NM_006144.3
 
 cDNA Size:   789
 
 Synonym:  HFSP, CTLA3
 
 Source:  Human.
 
Description 
 
cDNA Description:  ORF Clone of Homo sapiens granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) DNA.
 
 Vector:  Commercial pMD vector.(Produced by TAKARA. 
 More information: Please Inquire us)
 
 Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for a point mutation 462G/C not resulting in amino acid variation.
 
Shipping
 
5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature. 
 It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
 
Usage Protocol
 
 
Remove the marked edge in the center of the spot .
 
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
 
Transfer the tube to a heating block at 95oC for 15–30 min .
 
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap, the sample approximately 60 times.
 
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
 
 
cDNA Sequence and Quality Control 
 
基因序列:
 
 
 1 ATGAGGAACT CCTATAGATT TCTGGCATCC TCTCTCTCAG TTGTCGTTTC TCTCCTGCTA 61 ATTCCTGAAG ATGTCTGTGA AAAAATTATT GGAGGAAATG AAGTAACTCC TCATTCAAGA 121 CCCTACATGG TCCTACTTAG TCTTGACAGA AAAACCATCT GTGCTGGGGC TTTGATTGCA 181 AAAGACTGGG TGTTGACTGC AGCTCACTGT AACTTGAACA AAAGGTCCCA GGTCATTCTT 241 GGGGCTCACT CAATAACCAG GGAAGAGCCA ACAAAACAGA TAATGCTTGT TAAGAAAGAG 301 TTTCCCTATC CATGCTATGA CCCAGCCACA CGCGAAGGTG ACCTTAAACT TTTACAGCTG 361 ACGGAAAAAG CAAAAATTAA CAAATATGTG ACTATCCTTC ATCTACCTAA AAAGGGGGAT 421 GATGTGAAAC CAGGAACCAT GTGCCAAGTT GCAGGGTGGG GGAGGACTCA CAATAGTGCA 481 TCTTGGTCCG ATACTCTGAG AGAAGTCAAT ATCACCATCA TAGACAGAAA AGTCTGCAAT 541 GATCGAAATC ACTATAATTT TAACCCTGTG ATTGGAATGA ATATGGTTTG TGCTGGAAGC 601 CTCCGAGGTG GAAGAGACTC GTGCAATGGA GATTCTGGAA GCCCTTTGTT GTGCGAGGGT 661 GTTTTCCGAG GGGTCACTTC CTTTGGCCTT GAAAATAAAT GCGGAGACCC TCGTGGGCCT 721 GGTGTCTATA TTCTTCTCTC AAAGAAACAC CTCAACTGGA TAATTATGAC TATCAAGGGA 781 GCAGTTTAA
 
 

 Quality Control:   cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
官网链接：http://www.sinobiological.com/Granzyme-A-GZMA-cDNA-Clone-g-922.html