Catalog Number:  26920

Gene Symbol:   RAB3

Description:   Anti-Active Rab3 Mouse Monoclonal Antibody

Background:   Rab3 is a member of the Rab-family GTPases and is specifically targeted to synaptic vesicles.

Immunogen:   Recombinant full length protein of active Rab3A

Applications:   IP, IHC, IF

Recommended Dilutions:    1 µg for ~2 mg total cellular proteins for IP 1:100 dilution for IHC, IF

Concentration:   1 mg/ml

Host:   mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:   IgG

Purity:   Purified from ascites

Storage buffer:

Preservative:  no

Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Anti-active Rab3 antibody recognizes active Rab3 proteins of vertebrates.

Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:

Rab3 activation assay. Purified full-length Rab3A proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab3 monoclonal antibody (Cat. No. 26920). Immunoblot was with an anti-Rab3A polyclonal antibody (Cat. No. 21041).

Publications:

1.  VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors     Hepatology Volume 61, Issue 1, pages 361–374, January 2015

2.  Rab5 Activity Regulates GLUT4 Sorting into ***-Responsive and Non-***-Responsive Endosomal Compartments: A Potential Mechanism for Development of *** Resistance     Endocrinology. 2014 Sep;155(9):3315-28