美国产品商标: neweastbioBrand,null 抗体货号： 26913Item#, Anti-Active Ral Mouse Monoclonal Antibody<br>英文名称： Anti-Active Ral Mouse Monoclonal Antibody

Active Ral-GTP [26913]
[30 µL]

Catalog Number: 26913

Gene Symbol: Ral

Description: Anti-Active Ral Mouse Monoclonal Antibody

Background: The Ras-like small G proteins, RalA and RalB, are important components of Ras signaling pathways, implicated in the initiation and maintenance of tumorigenic transformation, as well as vesicle transport, apoptosis, transcription, cell migration, and cell proliferation.

Immunogen: Recombinant full length protein of active RalB.

Applications: IP, IHC

Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP;
1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Ral antibody recognizes active RalA and RalB of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:

Ral activation assay. Purified RalB proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Ral monoclonal antibody (Cat. No. 26913). Immunoblot was with an anti-RalB rabbit polyclonal antibody (Cat. No. 21033).