Active Rheb Mouse Monoclonal Antibody
英文名称： Anti-Active Rheb Mouse Monoclonal Antibody
型号:null    抗体货号： 26910
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

Active Rheb-GTP [26910]
[30 µL]		  

Catalog Number:  26910
Gene Symbol:  RHEB
Description:   Anti-Active Rheb Mouse Monoclonal Antibody
Background:   Small GTPases are a super-family of cellular signaling regulators. Rheb is a member of the Ras-superfamily GTPases. Rheb has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related (ATR).
Immunogen:   Recombinant full length protein of active Rheb.
Applications:   IP, IHC
Recommended Dilutions:   1 μg for 1~2 mg total cellular proteins for IP
1:100 dilution for IHC
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgM
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active Rheb antibody recognizes active Rheb of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Immunoprecipitation/Western blot:  
Rheb activation assay. Purified Rheb proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rheb monoclonal antibody (Cat. No. 26910). Immunoblot was with an anti-Rheb rabbit polyclonal antibody (Cat. No. 21098).

 

Publications:
1.  Inducible Nitric Oxide Synthase Drives mTOR Pathway Activation and Proliferation of Human Melanoma by Reversible Nitrosylation of TSC2
    Cancer Res. 2014 Feb 15;74(4):1067-78
2.  SynGAP Regulates Protein Synthesis and Homeostatic Synaptic Plasticity in Developing Cortical Networks
    PLoS One. 2013 Dec 31;8(12):e83941