Active RhoA Mouse Monoclonal Antibody
英文名称： Anti-Active RhoA Mouse Monoclonal Antibody
型号:null    抗体货号： 26904
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

Active RhoA -GTP [26904]
[30 µL]		  

Catalog Number:  26904
Gene Symbol:  RHOA
Description:   Anti-Active RhoA Mouse Monoclonal Antibody
Background:   Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction.
Immunogen:   Recombinant full length protein of active RhoA
Applications:   IP, IHC
Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP;
                                                   1:100 dilution for IHC
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG2b
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active RhoA antibody recognizes active RhoA from vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Immunoprecipitation/Western Blot:  
IP/WB analysis of active RhoA protein. Purified recombinant RhoA proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-active RhoA mouse monoclonal antibody (Cat. # 26904). After SDS/PAGE, the membrane filter was probed with anti-RhoA mouse monoclonal antibody (Cat. # 26007).
 

 

Publications:
1.  Ethanol increases p190RhoGAP activity, leading to actin cytoskeleton rearrangements
    J Neurochem. 2011 Dec;119(6):1306-16
2.  The transcriptional coactivators megakaryoblastic leukemia 1/2 mediate the effects of loss of the tumor suppressor deleted in liver cancer 1
    Oncogene. 2012 Aug 30;31(35):3913-23
3.  G(alpha)12/13 inhibition enhances the anticancer effect of bortezomib through PSMB5 downregulation
    Carcinogenesis. 2010 Jul;31(7):1230-7
4.  Transgenesis of the Wolffian duct visualizes dynamic behavior of cells undergoing tubulogenesis in vivo
    Development, Growth & Differentiation. Volume 55, Issue 4, pages 579–590, May 2013
5.  Foxc2 induces Wnt4 and Bmp4 expression during muscle regeneration and osteogenesis
    Cell Death and Differentiation (2013) 20, 1031–1042
6.  N-glycosylation induces collagen triple helix repeat containing 1 (CTHRC1) and drives oral cancer cell migration
    J Biol Chem. 2013 Jul 12;288(28):20217-27
7.  Cyclodextrin protects podocytes in diabetic kidney disease
    Diabetes. 2013 Nov;62(11):3817-27
8.  Amyloid beta1-42 (Aβ42) up-regulates the expression of sortilin via the p75NTR/RhoA signaling pathway
    Journal of Neurochemistry Volume 127, Issue 2, pages 152–162, October 2013
9.  Podocyte-specific GLUT4 deficient mice have fewer and larger podocytes and are protected from diabetic nephropathy
    Diabetes. 2014 Feb;63(2):701-14
10.  Loss of TAK1 increases cell traction force in a ROS-dependent manner to drive epithelial–mesenchymal transition of cancer cells
    Cell Death and Disease (2013) 4, e848
11.  Syndecan-2 regulation of morphology in breast carcinoma cells is dependent on RhoGTPases
    Biochimica et Biophysica Acta (BBA) - General Subjects Volume 1840, Issue 8, August 2014, Pages 2482–2490
12.  Epithelial repair is a two-stage process driven first by dying cells and then by their neighbours
    J Cell Sci. 2014 Mar 15;127(Pt 6):1229-41
13.  MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells
    Cytokine Volume 69, Issue 1, September 2014, Pages 47–55
14.  Triclosan Potentiates Epithelial-To-Mesenchymal Transition in Anoikis-Resistant Human Lung Cancer Cells
    PLoS One. 2014 Oct 16;9(10):e110851