Anti-Orai1 Rabbit Polyclonal Antibody (Orai1-L2)
英文名称：  Anti-Orai1 Rabbit Polyclonal Antibody (Orai1-L2)
型号:null    抗体货号： 21002
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

Orai1-L2 [21002]
[100 µL]		  

Catalog Number:  21002
Gene Symbol:  Orai1
Description:   Anti-Orai1 Rabbit Polyclonal Antibody (Orai1-L2)
Background:   Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a ~35 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.
Immunogen:   A peptide from the extracellular loop 2 region of human Orai1
Applications:   ELISA, WB
Recommended Dilutions:   
ELISA:      1:1000-1:5000,
WB:          1:1000-1:2000,
IHC:          1:100-1:200
Concentration:   
Host:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   IgG
Purity:   Immunogen affinity purified
Storage buffer:
Preservative:  0.01% sodium azide
Constituents:   50mM NaHCO3-Glycine, 0.15M NaCl,, pH7.4
Species Reactivity:  Orai1-L2 antibody recognizes Orai1 from human.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of Orai1 protein. Human Orai1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blot in the absence (A) or presence (B) of antigen peptide.

 

Publications:
1.  Generation and Characterization of Fully Human Monoclonal Antibodies Against Human Orai1 for Autoimmune Disease
    J Pharmacol Exp Ther. 2013 May;345(2):225-38
2.  Influence of Orai1 intervention on mouse airway epithelium reactions in vivo and in vitro
    Ann Allergy Asthma Immunol. 2012 Feb;108(2):103-12
3.  2-Aminoethoxydiphenyl borate administration into the nostril alleviates murine allergic rhinitis
    Am J Otolaryngol. 2011 Jul-Aug;32(4):318-28
4.  Up-regulation of Orai1 in murine allergic rhinitis
    Histochem Cell Biol. 2010 Jul;134(1):93-102
5.  Orai1 and STIM1 are critical for breast tumor cell migration and metastasis
    Cancer Cell. 2009 Feb 3;15(2):124-34
6.  Amelioration of Muc5b mucin hypersecretion is enhanced by IL-33 after 2-APB administration in a murine model of allergic rhinitis
    Biotech Histochem. 2014 May;89(4):273-86