L羟脯氨酸检测试剂盒
型号:null    产品货号： 238HYP01
价格:请致电：010-57128832,18610462672
品牌: 美国

Helix L-羟脯氨酸检测试剂盒可特异检测组织，牛奶， 肉制品等中的羟脯氨酸或蛋白质/多肽水解产物，也可以用来检测其他生物样品如净化过的血清或尿液中的L-羟脯氨酸。试样经酸水解， 释放出L-羟脯氨酸，经氯胺T氧化，生成含有***环氧化物，用高**破坏过量的氯胺T，L-羟脯氨酸氧化物与二甲氨基***甲醛反应生成红色化合物，这种检测方法简单方便，显色结果在560nm有最大吸光值。试剂盒检测原理与目前农业部公布的标准检测方法相同，但操作更加简便，大大缩短了检测时间，5小时内即可完成检测。检测范围是2 5 p p m ~ 4 0 0 p p m （ 牛奶） ；125ppm~2000ppm（奶粉）。

Gene Symbol:  SUMO1 Description:   Anti-SUMO-1 Rabbit polyclonal Antibody Background:   Small Ubiquitin-like Modifier or SUMO proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2 and -3 show a high degree of similarity to each other and are distinct from SUMO-1. Immunogen:   A synthetic peptide corresponding to residues near the C-terminal of human SUMO-1. Applications:   ELISA, WB, IHC, IP Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100 Concentration:   1 mg/ml Host:   Rabbit Format:   Liquid Clonality:   Polyclonal Isotype:    Purity:   Purified from serum Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Detects SUMO-1 but not SUMO-2/3 from vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles   Western blot:  
Western blot analysis of over-expressed SUMO-1 proteins. HEK293 cells were transfected with SUMO-1 (lane 2) and SUMO-2 (lane 3) cDNAs and control empty vector (lane 1). Cell lysates were blotted with anti-SUMO-1 rabbit polyclonal antibody (Cat. # 21236).

 

Publications:
1.  The SUMOylation of zinc-fingers and homeoboxes 1 (ZHX1) by Ubc9 regulates its stability and transcriptional repression activity
    Journal of Cellular Biochemistry Volume 114, Issue 10, pages 2323–2333, October 2013
2.  C-terminal Src Kinase (Csk)-mediated Phosphorylation of Eukaryotic Elongation Factor 2 (eEF2) Promotes Proteolytic Cleavage and Nuclear Translocation of eEF2
    J Biol Chem. 2014 May 2;289(18):12666-78