产品:人间质干细胞成脂肪细胞检测试剂盒 货号:8298 Description Human mesenchymal stem cells (MSC) are a population of multipotent cells that can be differentiated into multiple lineage-specific cells, which can form bone, fat, cartilage, muscle and tendon. The process of adipogenesis that forms fat plays a role in obesity, cardiovascular diseases and metabolic disorders. ScienCell has created a convenient multiplex PCR kit for the routine detection of human mesenchymal stem cell adipogenesis. Multiplex PCR allows two or more primers to be amplified in a single PCR reaction by using multiple primer pairs in a single reaction mixture, allowing for considerable saving in labor, cost and precious DNA samples. All required PCR reagents are supplied in this kit. Simply add DNA template and perform PCR reaction. Tube 1 ready-mix reaction contains PPARγ and FABP4 primers that allow for the detection of mid- and late-stage of human MSC adipogenesis, respective [1]. Product Use HMSC-A PCR kit is for research use only. Not for use in animals, humans, or diagnostic procedures. Storage Store at -20°C upon receipt. Avoid repeated freeze thaw cycles by making six aliquots at 110 μL each. Shipping Dry ice. Reference [1] Menssen, A. et al. (2011). Differential gene expression profiling of human bone marrow-derived mesenchymal stem cells during adipogenic development. BMC Genomics. 12: 461-477. |