英文名称： TNS
北京裕恒丰科技有限公司，已成功代理美国Sciencell公司细胞生物学产品十余年。多年来，裕恒丰一直秉承信誉至上的原则，在点滴中慢慢地提升企业信誉，树立企业形象，我们始终以热心的服务和专业的技术支持，为广大科研工作者提供了优质的细胞及分子生物学产品。在未来的发展中，我们将继续努力打造企业品牌，北京裕恒丰不仅希望您能聆听到我们的心声，更期待您能亲自体验我们的服务，北京裕恒丰欢迎您的光临。
温馨提示：ScienCell产品仅用于科研，不能用于人或动物以及体外诊断应用。如预购买产品请登陆ScienCell唯一官网（www.sciencellonline.com）寻找正规代理商，以确保您的实验顺利进行。
胰酶中和液 货号0113
产品介绍
Trypsin Neutralization Solution (TNS) is a sterile, phosphate and HEPES-buffered saline solution. It contains 10% fetal bovine serum as a trypsin inhibitor and cell protect agents. The product is calcium- and magnesium-free and has a pH of 7.4 at room temperature.
步骤
Incubating cells with too high a trypsin concentration for too long a time period will damage cell membranes and kill the cells. If unsure about the concentration of trypsin to use, use a low concentration. The time required to remove cells from the culture surface is dependent on cell type, population density, and serum concentration in the growth medium. The time of trypsin exposure should be kept to a minimum.
1) Remove medium from culture vessel by aspiration and wash the monolayer with Ca+2 and Mg+2-free salt solution to remove all traces of serum. Remove salt solution by aspiration.
2) Dispense enough trypsin/DTA solution into culture vessel to completely cover the
monolayer of cells and place in 37°C incubator for approximately 2 minutes or until 80% of cells are rounded up (monitored with microscope).
3) Remove the trypsin/EDTA solution by aspiration and return closed culture vessel to
incubator for 1 minute.
4) Add TNS or medium containing serum to the cells as soon as possible to inhibit further tryptic activity which may damage cells.
5) Cells can be harvested by gently pipetting the cell suspension. Further dilution can be made, if required, for cell counts and/or subculturing.
Caution: If handled improperly, some components of this product may present a health hazard. Take appropriate precautions when handling this product, including the wearing of protective clothing and eyewear. Dispose of properly.
