背景资料
Cal-520荧光染料为高通量检测细胞内钙离子流动提供了荧光分析方案。作为一款新的钙敏感的荧光染料，Cal-520 AM与目前的绿色钙离子指示剂，如Fluo-3 AM and Fluo-4 AM相比，具有更高的信噪比和细胞内留存。进入表达GPCR或者钙离子通道蛋白的细胞在加入Cal-520 AM后，后者由于具有疏水性的AM基团可以轻易穿透活细胞膜。当其进入到细胞质后，酯酶会将其水解为Cal-520留在细胞内，与细胞内钙离子结合后的Cal-520发出强绿色荧光（荧光强度增强100倍）。由于Cal-520的长波段覆盖、高灵敏度和超强激发，再加上很高的信噪比与细胞内留存，使其成为评估GPCR和钙离子通道研究的卓越工具，也可以用于其拮抗剂和抑制剂的筛选。

产品描述
Cal-520 No Wash Calcium Assay Kits provide homogeneous fluorescence-based assays for detecting intracellular calcium mobilization· Cal-520 AM is a new colorless fluorescent calcium-sensitive dye with a significantly improved signal to noise ratio and intracellular retention· Cells expressing a GPCR or calcium channel of interest that signals through calcium are pre-loaded with Cal-520 AM which can cross cell membrane· Once inside the cell, the lipophilic blocking groups of Cal 520 AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells· Its fluorescence is greatly enhanced upon binding to calcium· When cells stimulated with agonists, the receptor signals the release of intracellular calcium, which significantly increase the fluorescence of Cal-520· The characteristics of its long wavelength, high sensitivity, and >100 times fluorescence enhancement, make Cal-520 AM an ideal indicator for the measurement of cellular calcium· The high S/N ratio and better intracellular retention make the Cal-520 calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists· Cal-520 No Wash Calcium Assay Kits provide an optimized assay method for monitoring the GPCRs and calcium channels· The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation·

保存建议
对于 Cal-520 钙检测试剂盒 *去除培养基 尤其适合于难检测细胞系* 产品，我们推荐使用室温进行运输。在您收到货物后，我们建议在低于-15℃温度下冷冻保存，要求储存环境干燥并避免光照。产品有效期为6个月。