17-0980-01 | BUTYL SEPHAROSE 4 FAST FLOW 200ML | 17-0980-10 | BUTYL SEPHAROSE FAST FLOW, 25ML | 17-1014-01 | Q SEPHAROSE HIGH PERF. 75 ML | 17-1082-01 | PHENYL SEPHAROSE HIGH PERF. 75 | 17-1087-01 | SP SEPHAROSE HIGH PERF. 75 ML | 17-1273-01 | SOURCE 30S, 50 ML | 17-1275-01 | SOURCE 30Q, 50 ML | 17-1287-01 | ANX SEPH 4 FF HIGH SUB 500ML | 17-1287-10 | ANX SEPHAROSE FF HIGHSUB 25 ML | 17-5072-01 | Q SEPHAROSE XL, 300 ML | 17-5073-01 | SP SEPHAROSE XL, 300 ML | 17-5316-02 | Capto Q 100 ml | 17-5316-10 | CAPTO Q, 25 ML | 17-5317-02 | Capto MMC 100ml | 17-5317-10 | CAPTO MMC, 25ML | 17-5432-01 | Butyl Sepharose HP, 25 ml | 17-5432-02 | Butyl Sepharose HP, 200ml | 17-5437-01 | Q SEPHAROSE XL VIRUS LIC 300ML | 17-5437-10 | Q SEPHAROSE XL VIRUS LIC 25 ML | 17-5441-01 | CAPTO S, 100 ML | 17-5441-10 | CAPTO S, 25 ML | 17-5443-01 | CAPTO DEAE, 100 ML | 17-5443-10 | CAPTO DEAE, 25 ML | 17-5444-10 | CAPTO adhere 25 ML | Butyl/Octyl Sepharose™ 4 Fast Flow - Aliphatic hydrophobic interaction chromatography media made of highly cross-linked 90-μm agarose beads derivatized via uncharged, chemically stable ether linkages.
- The butyl derivative is the standard aliphatic medium; the octyl derivative gives different and complementary selectivity.
Butyl/Octyl Sepharose™ 4 Fast FlowTechnical Information
TECHNICAL SPECIFICATIONS | Ligand density | | Butyl Sepharose™ 4 Fast Flow | 40 μmol/ml medium | Octyl Sepharose™ 4 Fast Flow | 5 μmol/ml medium | Binding capacity | Butyl Sepharose™ 4 Fast Flow | 7 mg IgG/ml medium | Octyl Sepharose™ 4 Fast Flow | 27 mg HSA/ml medium | Average particle size | 90 μm | Pressure/flow spec. | min 150 cm/h, 1 bar, XK50/60 column, bed height 25 cm | pH stability | 2-14 (short term), 3-13 (long term) | Chemical stability | Stable in common buffers, chaotropic agents, detergents, and polar organic solvents. | Storage | 20% ethanol | Storage temperature | 4°C to 30°C | For additional technical specifications for Butyl and Octyl Sepharose™ 4 Fast Flow , see Introduction to Hydrophobic Interaction. | Separation pattern of a cell culture supernatant after a one-step separation with Butyl Sepharose™ 4 Fast Flow
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