17-1178-01 | RESOURCE S, 1 ML |
17-1180-01 | RESOURCE S, 6 ML |
SOURCE™ Media/RESOURCE™ and Tricorn Columns for IEX
Technical Information
SOURCE™ Media, RESOURCE™, and Tricorn prepacked ion exchange columns increase productivity.
TECHNICAL SPECIFICATIONS |
SOURCE media |
Ion exchanger type |
SOURCE™ 15Q, 30Q | Quaternary ammonium strong anion |
SOURCE™ 15S, 30S | Methyl sulfonate strong cation |
Particle size |
SOURCE™ 15Q, 15S | 15 μm |
SOURCE™ 30Q, 30S | 30 μm |
Matrix | Polystyrene/divinylbenzene |
pH stability | 2-12 (long term), 1-14 (short term) |
Chemical stability | Stable in all common ion exchange buffers |
Storage | 20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (S) |
Storage temperature | 4°C to 30°C |
RESOURCE™ prepacked columns |
Bed volume | 1 ml or 6 ml |
Bed diameter | 6.4 mm (1-ml column) 16 mm (6-ml column) |
Bed height | 30 mm |
Max. pressure | 1-ml column: 15 bar (218 psi, 1.5 MPa) 6-ml column: 6 bar (87 psi, 0.6 MPa) |
Max. flow rate* | 1800 cm/h (10 ml/min for 1-ml column) 1800 cm/h (60 ml/min for 6-ml column) |
SOURCE 4.6/100 PE columns (Tricorn)† |
Bed volume | 1.7 ml |
Bed dimensions | 4.6 mm × 100 mm |
Max. pressure | 40 bar (580 psi, 4 MPa) |
Max. flow rate* | 5 ml/min (1800 cm/h) |
* H2O at 25°C † Columns not suitable for use with ÄKTAprime™ plus system. |
Four different pH-values were tested for high resolution separation of recombinant protein DnaK.
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