英文名称： HIS SPINTRAP
| 28-9546-56 | PGEX-1LT ECOR I/BAP |
| 11-0033-99 | HIS GRAVITRAP |
| 11-0034-00 | HIS BUFFER KIT |
| 27-4710-01 | ANTI-HIS ANTIBODY |
| 28-4009-89 | HIS MULTITRAP HP |
| 28-4009-90 | HIS MULTITRAP FF |
| 28-4013-53 | HIS SPINTRAP |
| 28-4039-43 | COLLECTION PLATE |
| 28-9321-71 | His SpinTrap Kit |
| 27-1583-01 | LIGHT PRIMER MIX |
| 27-1586-01 | HEAVY PRIMER MIX |
| 27-1588-01 | LINKER-PRIMER MIX |
| 27-1589-01 | RS PRIMER MIX |
| 27-9420-01 | ANTI-M13 MONOCLONAL ANTIBODY |
| 27-9421-01 | HRP/ANTI-M13 MONOCLNL CONJUG. |
His SpinTrap/His SpinTrap Kit - Minipreps
- For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
- Ni Sepharose™ High Performance in prepacked spin columns.
- Yields up to 750 μg pure protein per column.
- Allows direct purification of both clarified and unclarified cell lysates
His SpinTrap/His SpinTrap Kit - Minipreps
Technical Information
* For licensing information, see Legal Info.
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His SpinTrap is a single-use spin column for purification of histidine-tagged proteins. For optimal convenience, use His SpinTrap Kit, which contains columns and buffers.
His SpinTrap columns
His SpinTrap™ is designed for efficient, small-scale purification of histidine-tagged proteins directly from clarified or unclarified cell lysates. The columns are also used for screening of large numbers of small-scale bacterial lysates, as well as optimization of purification conditions.
Purification of unclarified samples on His SpinTrap™ columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.
His SpinTrap columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap together with buffers prepared using His Buffer Kit, see His Buffer Kit - Convenient, Pre-made Buffers.
The columns are prepacked with Ni Sepharose High Performance (see Ni Sepharose™ High Performance - High-performance Purification), which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose High Performance medium is negligible.
His SpinTrap Kit
The benefits of His SpinTrap and His Buffer Kit are combined in His SpinTrap Kit, which contains one pack of His SpinTrap with 50 columns and one pack of His Buffer Kit. For more information about His Buffer Kit, see His Buffer Kit - Convenient, Pre-made Buffers
For the full range of products for purification of histidine-tagged proteins, see Introduction to Tagged Protein Purification.
 of unclarified and clarified<LineBreak/><italic>E. coli</italic> lysate containing GFP-(His)<sub>6</sub> purified using His SpinTrap. Similar purity and recovery was observed from both the unclarified and clarified sample (lanes 4<LineBreak/>and 5).))
SDS-PAGE (ExcelGel SDS Gradient 8-18) of unclarified and clarified E. coli lysate containing GFP-(His)6 purified using His SpinTrap. Similar purity and recovery was observed from both the unclarified and clarified sample (lanes 4 and 5).
 After placing the column in a 2<UNspace/>ml microcentrifuge tube, equilibrate by adding binding buffer and centrifuge<para/>(2) Add sample, centrifuge<para/>(3) Wash with binding buffer, centrifuge<para/>(4) Elute the target protein with elution by centrifugation))
Purifying histidine-tagged proteins with His SpinTrap is a simple four-stage procedure that can be performed in 10 min using a microcentrifuge:
(1) After placing the column in a 2 ml microcentrifuge tube, equilibrate by adding binding buffer and centrifuge
(2) Add sample, centrifuge
(3) Wash with binding buffer, centrifuge
(4) Elute the target protein with elution by centrifugation
| TECHNICAL SPECIFICATIONS | |
| Medium | Ni Sepharose High Performance |
| Average particle size | 34 μm |
| Max. sample loading volume | 600 μl |
| Bed volume | 100 μl |
| Protein binding capacity* | Approx. 750 μg histidine-tagged protein/column |
| Compatibility during use | Stable in all commonly used buffers, reducing agents, denaturants and detergents, see Introduction to Tagged Protein Purification for more information. |
| Storage | 0.15% Kathon CG |
| Storage temperature | 4°C to 30°C |
| Column material | Polypropylene barrel and polyethylene frits |
| * Binding capacity is protein-to-protein dependent | |
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