Anti-KIT (W557R) Mouse Monoclonal Antibody
英文名称： Anti-KIT (W557R) Mouse Monoclonal Antibody
型号:null    抗体货号： 26275
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

KIT (W557R) [26275]
[100ul]		  

Catalog Number:  26275
Gene Symbol:  C-Kit; CD117; KIT; PBT; SCFR
Description:   Anti-KIT (W557R) Mouse Monoclonal Antibody
Background:   Tyrosine-protein kinase Kit, also known as proto-oncogene c-Kit or CD117 or Mast/stem cell growth factor receptor (SCFR), is a receptor tyrosine kinase protein that in humans is encoded by the KIT gene. Activating mutations in this gene are associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, acute myeloid leukemia, while inactivating mutations are associated with the genetic defect piebaldism.
Immunogen:   A synthetic peptide from the internal region of KIT which includes the mutation of W557R, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IF: 1:50-1:100
IHC: 1:25-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes W557R mutant, but not wild-type KIT of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant KIT (W557R) and wildtype proteins. Purified His-tagged KIT (W557R) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-KIT (W557R) monoclonal antibody (Cat. #26275).
Immunofluorescence:  

 

Immunofluorescence of cells expressing KIT proteins with anti-KIT (W557R) antibody. HEK293T cells were transfected with pCDNA3-GFP-KIT (WT) plasmid (left column) or pCDNA3-GFP-KIT (W557R) plasmid (right column), then fixed and stained with anti-KIT (W557R) monoclonal antibody (Cat. #26275).