Anti-IDH2 Rabbit Polyclonal Antibody
英文名称： Anti-IDH2 Rabbit Polyclonal Antibody
型号:null    抗体货号： 21278
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

IDH2 [21278]
[100 µL]		  

Catalog Number:  21278
Gene Symbol:  IDH2
Description:   Anti-IDH2 Rabbit Polyclonal Antibody
Background:    Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
Immunogen:   Recombinant protein of human IDH2 (full length).
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:1000-1:5000 
IHC: 1:50-1:500
Concentration:    0.1mg/ml
Host:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   
Purity:   Purified from serum
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-IDH2 antibody recognizes IDH2 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
 
Western blot:  
Western blot analysis of recombinant IDH2 protein. Purified His-tagged IDH2 protein (full length) (Cat. #10200) was blotted with anti-IDH2 rabbit polyclonal antibody (Cat. # 21278).
Publications:
1.  Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
    Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11
2.  Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
    Nature 513, 110–114 (04 September 2014)
3.  Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
    Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015