Anti-SMAD4 (D351H) Mouse Monoclonal Antibody
英文名称： Anti-SMAD4 (D351H) Mouse Monoclonal Antibody
型号:null    抗体货号： 26418
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

SMAD4 (D351H) [26418]
[100]		  

Catalog Number:  26418
Gene Symbol:  SMAD4, DPC4, JIP, MADH4, MYHRS
Description:   Anti-SMAD4 (D351H) Mouse Monoclonal Antibody
Background:   SMAD4 family member 4, also known as SMAD4, is a protein that in human is encoded by the SMAD4 gene. It is often found mutated in many cancers. It acts as a tumor suppressor that functions in the regulation of the TGF-? signal transduction pathway, which negatively regulates growth of epithelial cells and the extracellular matrix (ECM). SMAD4 alterations have been found in multiploid colorectal cancer and pancreatic carcinoma. It is found inactivated in at least 50% of pancreatic cancers. It is also found mutated in the autosomal dominantdisease juvenile polyposis syndrome (JPS).
Immunogen:   A synthetic peptide from the internal region of SMAD4 which includes the mutation of D351H, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes D351H mutant, but not wild-type SMAD4 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant SMAD4 (D351H) and wildtype proteins. Purified His-tagged SMAD4 (D351H) protein (lane1) and corresponding wild-type protein (1-552aa, 65kd) (lane2) were blotted with anti-SMAD4 (D351H) monoclonal antibody (Cat. #26418).
Immunofluorescence:  
Immunofluorescence of cells expressing SMAD4 proteins with anti-SMAD4 (D351H) antibody. HEK293T cells were transfected with pCDNA3-GFP-SMAD4 (D351H) plasmid , pCDNA3-GFP-SMAD4 (WT) plasmid or pCDNA3-GFP-SMAD4 (D351N) plasmid, then fixed and stained with anti-SMAD4 (D351H) monoclonal antibody (Cat. #26418).