Anti-Kim-1 Rabbit Polyclonal Antibody
英文名称: Anti-Kim-1 Rabbit Polyclonal Antibody
型号:null    抗体货号: 21110
价格:请致电:010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

Kim-1 [21110]
[100 µL]
 

Catalog Number:  21110
Gene Symbol:  Kim-1
Description:   Anti-Kim-1 Rabbit Polyclonal Antibody
Background:   Kidney injury molecule-1 (Kim-1) is a type 1 membrane protein with extracellular immunoglobulin and mucin domains. Kim-1 mRNA and protein are expressed at very low levels in normal kidney, but expression increases dramatically after injury in proximal tubule epithelial cells in postischemic rodent kidney and in humans during ischemic acute renal failure.
Immunogen:   A synthetic peptide from the internal region of Kim-1 of human origin
Applications:   ELISA, WB, IHC, IP
Recommended Dilutions:   
ELISA: 1:100-1:5000
WB: 1:200-1:1000
IHC: 1:50-1:100
Concentration:   0.2 mg/ml
Host:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   IgG
Purity:   Purified from serum
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Kim-1 antibody recognizes Kim-1 from vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  

 

HEK 293T cell lysate was used for western blot with anti-Kim-1 polyclonal antibody (Cat # 21110).

i-font-family: Times New Roman">表达载体

11905ES03

1µg

-20

788.00

产品描述

pET系列载体是目前应用最广泛的重组蛋白表达载体,载体构建时目的片段被克隆入载体噬菌体T7转录翻译系统下,经转化在宿主T7RNA聚合酶的诱导下进行表达。T7RNA聚合酶具有超强并特异的启动T7启动子基因表达功能,当其被完全诱导时,可使宿主本身的表达几乎全部转化为目的基因的表达,诱导几个小时后即可使得最终目的基因表达产物超过细胞总蛋白的50%

pET-28a载体带有一个N端的His/Thrombin/T7蛋白标签,同时含有一个可以选择的CHis标签。pET28a载体的单一的多克隆位方便目的片段的克隆。

产品性质

质粒类型(Plasmid Type

细菌表达

表达水平(Expression Level

大小(size

5369

5测序引物(5 Sequencing 1 Primer

T7 Fwd

5测序引物序列(5 Sequencing 1 Primer Sequence

5d[TAATACGACTCACTATAGGG]3

抗性(Bacterial Resistance

Kanamycin

类型(Viral/Non-Viral

Nonviral

运输和保存方法

冰袋运输。≤-20℃保存,长期不用置于-80℃保存,避免反复冻融。

注意事项

1)载体序列是以pBR322质粒的编码原则进行编码的,所以T7蛋白表达区在质粒图谱上面是反向的

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。