ScienCells GeneQuery™ human melanocyte development & pigmentation qPCR array kit (GQH-MDP) is designed to facilitate gene expression profiling of key genes involved in melanocyte development and pigmentation pathways. 40 genes are selected in this kit based on public database and literature research.      

GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery™ Human Melanocyte Development & Pigmentation qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ADCY3

CTNNB1

GRB2

MC1R

PRKACA

β-actin

ADCY3

CTNNB1

GRB2

MC1R

PRKACA

β-actin

B

AKT1

EDNRB

GSK3B

MITF

QPCT

GAPDH

AKT1

EDNRB

GSK3B

MITF

QPCT

GAPDH

C

AKT3

EP300

HRAS

MLANA

RPS6KA1

LDHA

AKT3

EP300

HRAS

MLANA

RPS6KA1

LDHA

D

BCL2

FOXD3

KIT

OCA2

SOS1

NONO

BCL2

FOXD3

KIT

OCA2

SOS1

NONO

E

BRAF

FZD3

KITLG

PAX3

SOX10

PPIH

BRAF

FZD3

KITLG

PAX3

SOX10

PPIH

F

CDH1

FZD4

LEF1

PIK3CA

TRP2

GDC

CDH1

FZD4

LEF1

PIK3CA

TRP2

GDC

G

CREB1

GNB2L1

MAPK1

PMEL

TYR

PPC

CREB1

GNB2L1

MAPK1

PMEL

TYR

PPC

H

CREBBP

GPR143

MAPK3

POMC

TYRP1

NTC

CREBBP

GPR143

MAPK3

POMC

TYRP1

NTC

 

* gene selection may be updated based on new research and development