ScienCells GeneQuery™ human pluripotent stem cell qPCR array kit (GQH-hPSC) is designed to facilitate gene expression profiling of key genes involved in maintaining pluripotency and self-renewal of hPSC, and genes regulated during differentiation of hPSC. 88 genes are selected in this kit based on public database and literature research.      

GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Pluripotent Stem Cell qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

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2

3

4

5

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7

8

9

10

11

12

A

ACTC1

CDX2

EN2

FOXA2

GFAP

ISL1

MIXL1

NOG

PECAM1

SOX1

TBX6

β-actin

B

AFP

CER1

ENO2

FOXD3

GJA1

KLF4

MYF5

NPM1

POU5F1

SOX2

TCF2

GAPDH

C

ALB

COL1A1

EOMES

FOXJ3

GSC

LEFTYA

MYOD1

NR5A2

PTF1A

SOX7

TCF3

LDHA

D

ALDH1A1

COL2A1

ESG1

GATA2

HAND1

LEFTYB

NANOG

OLIG2

REST

SOX17

TDGF1

NONO

E

BRIX1

CXCR4

ESRRB

GATA4

HDAC2

LIN28

NEUROD

OTX2

REX1

T

TERT

PPIH

F

CD34

DES

FGF4

GATA6

HLXB9

MEF2C

NCAM1

PAX2

RUNX1

TAT

TUBB3

GDC

G

CD90

DNMT3B

FGF5

GBX2

HNF4A

MEIS1

NES

PAX6

RUNX2

TBX3

UTF1

PPC

H

CDH1

EMX2

FLT1

GDF3

IDH1

MESP1

NKX2.5

PDX-1

SALL4

TBX5

VIM

NTC

 

* gene selection may be updated based on new research and development