28-4055-00 GST MULTITRAP 4B

28-4055-01 GST MULTITRAP FF

28-9523-59 GST SpinTrap

28-9523-60 GST GraviTrap

28-9523-61 GST Buffer Kit

28-9545-49 PGEX-4T-1

28-9545-50 PGEX-4T-2

28-9545-52 PGEX-4T-3

28-9545-53 PGEX-5X-1

28-9545-54 PGEX-5X-2

28-9545-55 PGEX-5X-3

28-9546-46 PGEX-2TK

28-9546-48 PGEX-6P-1 VECTOR

28-9546-50 PGEX-6P-2 VECTOR

28-9546-51 PGEX-6P-3 VECTOR

28-9546-53 PGEX-2T 25 UG WI/DI

28-9546-54 PGEX-3X 25 UG WI/DI

28-9546-56 PGEX-1LT ECOR I/BAP

11-0033-99 HIS GRAVITRAP

11-0034-00 HIS BUFFER KIT

27-4710-01 ANTI-HIS ANTIBODY

28-4009-89 HIS MULTITRAP HP

28-4009-90 HIS MULTITRAP FF

28-4013-53 HIS SPINTRAP

28-4039-43 COLLECTION PLATE

28-9321-71 His SpinTrap Kit

27-1583-01 LIGHT PRIMER MIX

27-1586-01 HEAVY PRIMER MIX

27-1588-01 LINKER-PRIMER MIX

27-1589-01 RS PRIMER MIX

27-9420-01 ANTI-M13 MONOCLONAL ANTIBODY

27-9421-01 HRP/ANTI-M13 MONOCLNL CONJUG.

详情请查询以下网址：

http://www.gelifesciences.com/webapp/wcs/stores/servlet/productById/zh/GELifeSciences-us/28952360

GST GraviTrap

GST GraviTrap contains 10 disposable columns prepacked with 2 ml of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion protein

Convenient, prepacked gravity-flow columns.
Allows direct purification of both clarified and unclarified cell lysates.
Yields up to 20 mg of pure proteins per column.
Yields up to 20 mg of pure proteins per column

GST GraviTrap contains 10 disposable columns prepacked with 2 ml of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion protein. GST-tagged proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the proteins.

The binding capacity of prepacked GST GraviTrap columns, which can be greater than 10 mg protein/ml medium, varies with the nature of the tagged protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged proteins.

GST GraviTrap — Technical Specifications

Complete Packsize 10 x 2 ml

Bed Volume 2 ml

Binding Capacity/Column Up to 20 mg recombinant glutathione S-transferase (rGST)

Storage Conditions 4 to 30°C, 20% Ethanol

Media

Ligand Glutathione

Average Particle Size 90 μm

Matrix Agarose, 4%

Particle Size 45 µm-165 µm

Binding Capacity/ml Chromatography Medium > 25 mg horse liver GST/ml medium¹⁾

pH stability Working Range 4-13

pH stability Cleaning 4-13

Storage Conditions 4 to 30°C, 20% Ethanol

Chemical Stability All commonly used aqueous buffers

¹⁾Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.

Column

Column i.d. 15 mm

Chemical Stability Stable in all commonly used buffer systems

Material [Column Hardware] Polypropylene (PP)

Material [Bed Support] Polyethylene (PE)