ScienCells GeneQuery™ human cell cycle qPCR array kit (GQH-CCY) is designed to facilitate gene expression profiling of key genes involved in human cell cycle progression. 88 genes are selected in this kit based on public database and literature research.      

GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Cell Cycle qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ANAPC2

BUB3

CCND2

CDC20

CDK6

CHEK1

E2F1

HDAC1

MCM3

RAD1

SMAD2

β-actin

B

ATM

CASP3

CCND3

CDC25A

CDK7

CHEK2

E2F2

HDAC2

MCM4

RAD17

SMC3

GAPDH

C

ATR

CCNA1

CCNE2

CDC25C

CDK8

CKS1B

E2F4

HUS1

MCM5

RAD51

STAG1

LDHA

D

AURKB

CCNA2

CCNF

CDC34

CDKN1A

CKS2

E2F5

KNTC1

MDM2

RAD9A

TFDP1

NONO

E

BCCIP

CCNB1

CCNG1

CDC6

CDKN1B

CUL1

EP300

KPNA2

MYC

RB1

TFDP2

PPIH

F

BIRC5

CCNB2

CCNH

CDK1

CDKN2A

CUL2

GADD45A

MAD2L1

NBN

RBL1

TGFB1

GDC

G

BRCA1

CCNC

CCNT1

CDK2

CDKN2B

CUL3

GSK3B

MAD2L2

ORC1

RBL2

TP53

PPC

H

BRCA2

CCND1

CDC16

CDK4

CDKN3

DBF4

GTSE1

MCM2

PCNA

SKP2

WEE1

NTC

 

* gene selection may be updated based on new research and development