Biotinylated Human TNF-alpha, epitope tag free, ultra sensitivity (primary amine labeling)
英文名称： Biotinylated Human TNF-alpha, epitope tag free, ultra sensitivity (primary amine labeling)
型号:null    产品货号： TNA-H8211
价格:请致电：010-57128832,18610462672
品牌: usa
试剂级别： 试剂级

分子量:40.4 kDa纯度:>95% as determined by SDS-PAGE and more than 90% measured by SEC-HPLC.内毒素:Less than 1.0 EU per μg of the Human CD47, Fc Tag (HPLC-verified) by the LAL method.Buffer:50 mM tris, 100 mM glycine, pH7.5生物活性:Measured by its binding ability in a functional ELISA. Immobilized Human CD47, Fc Tag (HPLC-verified) (Catalog # CD7-H5256) at 2 μg/mL  can bind  Human SIRP alpha, His Tag (Catalog # SIA-H5225) with a linear range of 16-250 ng/mL.产品特性:Human CD47, Fc Tag (HPLC-verified) is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 40.4 kDa. The predicted N-terminus is Gln 19. The reducing (R) protein migrates as 50-65 kDa in SDS-PAGE  due to glycosylation.产品背景:Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
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border-left: windowtext 0.5pt solid; background-color: white; height: 13.5pt; border-top: windowtext; border-right: windowtext 0.5pt solid">11699083001 Nylon Membranes, 50 discs (132 50 discs (each 132 mm diameter) 11175050001 Restr.-Endonucl. Asp 718, conc 5,000 U (40 U/µl) 10109118001 Reverse Transcriptase AMV, 100 1,000 U  11785826001 ExpandTM Reverse Transcriptase 1,000 U (20 µl) 10822221001 Restr.-Endonucl. Mae I, 250 u 250 U (1 - 5 U/µl) 11274040001 Restr.-Endonucl.Hind III, conc 50,000 U (40 U/µl) 11097059001 Restr.-Endonucl. Bcl I, conc.  2,500 U (40 U/µl) 10835277001 Restr.-Endonucl. Asp 700 (Xmn  500 U (10 U/µl) 11362399001 Meganuclease I-Sce I 1,000 U  11464841001 Restr.-Endonucl. Pin AI, 200 u 200 U (10 U/µl) 10814253001 Restr.-Endonucl. Asp 718, 5000 5,000 U (10 U/µl) 11814389001 RED BLOOD CELL LYSIS BUFFER-CN 100 ml  11464825001 Restr.-Endonucl. Tru 9I, 1000  1,000 U (10 U/µl) 4823125001 High Pure FFPE Micro Kit 1 kit (up to 50 isolations) 11288075001 Restr.-Endonucl. Mva I         5,000 U (10 U/µl)  11117807001 Restr.-Endonucl. Ksp I         1,000 U (10 U/µl) 10693952001 Restr.-Endonucl. Bcl I, 500u 500 U (10 U/µl) 10843547001 Restr.-Endonucl. Dra III, 500u 500 U (1 - 5 U/µl) 11062573001 Restr.-Endonucl. Mvn I 200 U (10 U/µl) 10798983001 Restr.-Endonucl.Hind III, conc 10,000 U (40 U/µl) 11418165001 Biotin Protein Labeling Kit 1 kit (5 labeling reactions) 10909718001 Restr.-Endonucl. Mlu I, 2500 u 2,500 U (10 U/µl) 10814245001 Restr.-Endonucl. Asp 718, 1000 1,000 U (10 U/µl) 10835307001 Restr.-Endonucl. Dde I, 1000U 1,000 U (10 U/µl) 3553426001 FastStart High Fidelity PCR Sy 125 U  11585649910 Biotin High Prime   100ul 100 µl (25 reactions) 11585584001 High Prime DNA Labeling Kit    1 kit (50 labeling reactions) 11426346910 Anti-Fluorescein-POD 150 U  11484281001 BM Blue POD Substrate, soluble 100 ml  11332481001 Triton X-100, 5x10 ml 5 x 10 ml  10800058001 Storage-Box 1 box  4709691001 FuGENE HD Transfection Reagent 0.4 ml (up to 120 transfections) 11667009001 Strip PCR Tubes and Caps, 8 st 125 strips (8 tubes/strip)