HYBOND-N+
英文名称: HYBOND-N+
型号:: RPN303B
价格:请致电:010-57128832,18610462672
品牌: 美国

RPN119B HYBOND-N+, 50X11.9X7.8CM
RPN119N HYBOND-N, 50X11.9X7.8CM
RPN1210B HYBOND-N+,20X12CMX10CM
RPN1210N HYBOND-N, 20X12CMX10CM
RPN132B HYBOND-N+, 50X132MM DISCS
RPN132N HYBOND-N, 50X132MM DISCS
RPN132T HYBOND-NX NYLON MEMBRANE 50X1
RPN137B HYBOND-N+, 50X137MM DISCS
RPN137N HYBOND-N, 50X137MM DISCS
RPN137T HYBOND-NX NYLON MEMBRANE 50X1
RPN1510B HYBOND-N+, 20X15CMX10CM
RPN1510N HYBOND-N, 20X15CMX10CM
RPN1520B HYBOND-N+, 10X15CMX20CM
RPN1520N HYBOND-N, 20X15CMX20CM
RPN1520T HYBOND-NX NYLON MEMBRANE 10 X
RPN1732B HYBOND-N+, 50X132MM DISCS,GRID
RPN1737B HYBOND-N+, 50X137MM DISCS,GRID
RPN1782B HYBOND-N+, 50X82MM DISCS,GRIDD
RPN1787B HYBOND-N+, 50X87MM DISCS,GRIDD
RPN2020B HYBOND-N+, 10X20CMX20CM
RPN2020N HYBOND-N, 10X20CMX20CM
RPN2020T HYBOND-NX NYLON MEMBRANE 10 X
RPN203B HYBOND-N+, 20CMX3M
RPN203N HYBOND-N, 20CMX3M
RPN203T HYBOND-NX NYLON MEMBRANE 20CM
RPN2222B HYBOND-N+, 10X22CMX22CM
RPN2222N HYBOND-N, 10X22CMX22CM
RPN2222T HYBOND-NX NYLON MEMBRANE 10 X
RPN2250B HYBOND N+ 50 X 22.2 X 22.2 CM
RPN303B HYBOND-N+, 30CMX3M
RPN303N HYBOND-N, 30CMX3M
RPN303T HYBOND-NX NYLON MEMBRANE 30CM
RPN3050B HYBOND-N+, 30CMX50CM
RPN82B HYBOND-N+, 50X82MM DISCS
RPN82N HYBOND-N, 50X82MM DISCS
RPN82T HYBOND-NX NYLON MEMBRANE 50X8
RPN87B HYBOND-N+, 50X87MM DISCS
RPN87N HYBOND-N, 50X87MM DISCS

Amersham Hybond™-N+

  • Positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm2¾recommended for use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems.
  • Recommended for all high density membrane arrays.

Amersham Hybond™-N+

Technical Information


Amersham Hybond™-N+ yields excellent sensitivity in conventional Southern blotting. Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation is best for maximum reproducibility. Hybond-N+ is not suitable for Alkali blotting and fixation.

 

  • Multiple reprobing is possible without loss of membrane integrity.
     
  • QC tested in genomic Southern blots using GE Healthcare products.
     
  • Special sizes available on request.

 

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Human genomic DNA (1, 0.5, 0.125, 0.06 and 0.03 µg loadings) digested with HindIII and transferred to Hybond-N+. Hybridizaton with A. N-ras, B. p53, and C. bcl-2 labeled DNA probes and detected using Gene Images™ random-prime labeling with CDP-Star™ detection.

 

Membranes should be stored at room temperature in a clean dry atmosphere away from excessive heat, light, and noxious fumes. To prevent contamination, handle membranes using gloves or blunt-ended forceps. Hybond-N+ is stable for up to 3 years. Membranes should be kept in the bags in which they are received. Performance is consistent when stored under the recommended conditions.

 

TECHNICAL SPECIFICATIONS
Pore size  0.45 µm 
Nucleic acid size required  > 50 bp 

http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=46939

 

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细胞株名称:Babl/C鼠肺成纤维胞

代号:NIH3T3

培养液:1640 10%

细胞培养基本方法:
(一)准备和安装过滤器,(二)合成培养基的配制,(三)小牛血清的处理,(四)生长培养基的配制,(五)冻存细胞的复苏,(六)传代,(七)冻存

一:1:人正常细胞   2:肺部细胞系  3:肝部细胞系

   4:肾部细胞株    5:胃肠部细胞    6:真皮细胞

   7:脂肪细胞  &nb, sp;  8:视觉部位细胞系 9:尿道细胞系

   10:生殖细胞系  11:淋巴细胞系   12:骨细胞系

   13:骨骼肌细胞系 14:口部细胞系   15:毛发细胞系

,

   16:脐带细胞系  17:间叶干细胞系 18:内皮细胞

二: 动物细胞   

三:细胞培养相关试剂

 四:传统培养基 (1,传统培养基 2低血清培养基3:无血清培养基 4无动物成分培养基 )

 五:试剂

六;分子生物学试剂

七:细胞培养基.

八:动物血清  胎牛血清  新生牛血清 马血清  猪血清 鸡血清 羊血清

             兔血清   豚鼠血清   血琼脂平板培养基 牛血清白蛋白

             羊脱纤维全血  等

细胞株名称

代  号

培养液

细胞形态

鼠胚胎成纤维细胞

3T3-Swiss*

DMEM 10%

梭形

Babl/C鼠肺成纤维胞

NIH3T3

1640 10%

成纤维样

脂肪前体细胞

3T3-L1

DMEM 10%

小鼠成纤维浆细胞

L929

1640 10%

成纤维样

小鼠淋巴细胞

CTLL-2*

1640 10%

IL-2(1000U/ml)

圆形悬浮)

小鼠树突状细胞

DC2.4

1640 10%

小鼠单核细胞-巨噬胞

RAW264.7

DMEM 10%

小鼠胚胎

成纤维细胞

PA317

DMEM 10%