28-9412-79 Mammalian Cell Lysis Buffer

28-9435-22 Extraction Buffer Trial Kit

28-9435-23 2-D Protein Extraction BufferI

28-9435-24 2-D ProteinExtraction BufferII

28-9435-25 2-DProteinExtraction BufferIII

28-9435-26 2-DProteinExtraction Buffer IV

28-9435-27 2-DProteinExtractionBufferV

28-9435-28 2-DProteinExtractionBufferVI

28-9440-06 Protein A Mag Sepharose 1x500uL

28-9440-08 Protein G Mag Sepharose 1 x 50

28-9440-09 NHS Mag Sepharose 1x500ul

28-9440-10 TiO2 Mag Sepharose 1x500ul

28-9440-45 Yeast Buffer Kit

28-9466-03 HiTrap Albumin IgG Depl,2x1 ml

28-9480-20 Albumin IgG Depletion SpinTrap

28-9489-64 Mag Rack 6

28-9513-77 TiO2 Mag Sepharose 4x500ul

28-9513-78 Protein A Mag Sepharose 4x500u

28-9513-79 Protein G Mag Sepharose 4x500u

28-9513-80 NHS Mag Sepharose 4x500ul

Protein A Mag Sepharose

Protein A Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. Based on Sepharose with native protein A as ligand.

Visible and dense Sepharose based magnetic beads with protein A ligand, easy to spot and collect bound target protein
Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
Optimized capacity for enrichment/immunoprecipitation of target proteins - only low amounts of antibody needed
Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis

Protein A Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with native protein A as ligand. Protein A has high affinity for the Fc region of IgG antibodies from a broad variety of species

There are two protocols available for protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis

Protein A Mag Sepharose combines well-proven enrichment methods with the magnetic beads platform, which is highly suitable for small-scale sample preparation. Together with MagRxt; border-right: windowtext 0.5pt solid">MabSelect SuRe LX 25 ML

Technical Information

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Glutathone Sepharose 6 Fast Flow for purification of GST-tagged proteins.

Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.

For more information of the full range of products included in the Glutathione S-transferase (GST*) Gene Fusion System, see Introduction to Glutathione S-Transferase (GST) Gene Fusion System. A more comprehensive guide to the GST Gene Fusion System is available in handbook 18-1157-58, see Media Reference Books, Software and Video Cassettes.

TECHNICAL SPECIFICATIONS
Ligand	Glutathione
Ligand coupling method	Epoxy activation
Matrix	Highly cross-linked agarose, 4%
Average particle size	90 μm
Binding capacity*	» 10 mg recombinant GST/ml medium
pH stability	3-12
Chemical stability	All commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol, 6 M guanidine hydrochloride (1 h at room temperature)
Storage	20% ethanol
Storage temperature	4°C to 30°C
*Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.

Protein A Mag Sepharose

Glutathione Sepharose 4 Fast Flow - Scale-up Purification

Technical Information