英文名称： HiPrep 16/60 Sephacryl S-500 H
| 28-9356-05 | HiPrep 26/60 Sephacryl S-400 H |
| 28-9356-06 | HiPrep 16/60 Sephacryl S-500 H |
| 28-9356-07 | HiPrep 26/60 Sephacryl S-500 H |
| 28-9365-38 | HiPrep Q XL 16/10 |
| 28-9365-40 | HiPrep SP XL 16/10 |
| 28-9365-41 | HiPrep DEAE FF 16/10 |
| 28-9365-42 | HiPrep CM FF 16/10 |
| 28-9365-43 | HiPrep Q FF 16/10 |
| 28-9365-44 | HiPrep SP FF 16/10 |
| 28-9365-45 | HiPrep Phenyl FF (high sub) 16 |
| 28-9365-46 | HiPrep Phenyl FF (low sub) 16/ |
| 28-9365-47 | HiPrep Butyl FF 16/10 |
| 28-9365-48 | HiPrep Octyl FF 16/10 |
| 28-9365-49 | HiPrep Heparin FF 16/10 |
HiPrep™ Sephacryl™ HR Columns and Sephacryl™ Lab Packs
- Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl ***/ bisacrylamide matrix.
- Sephacryl™ S-100 HR is designed for separating peptides and small proteins.
- S-200 HR and S-300 HR are for purifying antibodies, serum proteins, and mid-size proteins.
- S-400 HR and S-500 HR are used to separate polysaccharides, macromolecules with extended structures, and even small plasmids.
- S-1000 SF is optimized for the purification of ***s up to 108 molecular weight, spherical particles up to 400 nm, and DNA up to 20 000 base pairs including plasmids, vesicles, and viruses.
- Convenient, prepacked HiPrep™ Sephacryl™ columns speed up protein purification.
HiPrep™ Sephacryl™ HR Columns and Sephacryl™ Lab Packs
Technical Information
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HiPrep™ Sephacryl™ HR columns, prepacked with Sephacryl™ High Resolution media, provide reliable gel filtration separations.
HiPrep™ Sephacryl™ HR prepacked gel filtration columns offer excellent separation properties in a convenient, ready-to-use format. By combining the performance of Sephacryl™ High Resolution media with a low cost column construction, they are both reliable and cost effective to use with simple equipment or systems.
Five HiPrep™ Sephacryl™ HR columns are available prepacked with S-100, S-200, S-300, S-400, or S-500 HR media, respectively. Each column is also available in two sizes: 16/60 for sample volumes of up to 5 ml and 26/60 for sample volumes of up to 13 ml. The range of HiPrep™ Sephacryl™ HR prepacked columns allows purification of molecules with molecular weights as small as 1 × 103 to macromolecules as large as 2 × 107.
| TECHNICAL SPECIFICATIONS | |
| Sephacryl™ High Resolution | |
| Fractionation range (Mr) - | |
| - Globular proteins | |
| S-100 HR S-200 HR S-300 HR S-400 HR | 1 × 103 - 1 × 105 5 × 103 - 2.5 × 105 1 × 104 - 1.5 × 106 2 × 104 - 8 × 106 |
| - Dextrans | |
| S-200 HR S-300 HR S-400 HR S-500 HR S-1000 SF | 1 × 103 - 8 × 104 2 × 103 - 4 × 105 1 × 104 - 2 × 106 4 × 104 - 2 × 107 5 × 105 - > 108 |
| Exclusion limit up to 400 nm for spherical particles. | |
| - DNA exclusion limit | |
| S-200 HR S-300 HP S-400 HR S-500 HR S-1000 SF | 30 bp 118 bp 271 bp 1078 bp 20 000 bp |
| Matrix | Spherical allyl *** and N, N’-methylenebisacrylamide |
| Chemical stability | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine-HCl, 1% SDS, 2 M NaCl, 24% ethanol, 30% propanol, 30% acetonitrile, 0.5 M NaOH (for cleaning) |
| pH stability | 3-11 (long term and working) 2-13 (short term) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| Physical stability Autoclavability Antimicrobial agent | Negligible volume variation due to changes in pH or ionic strength At 121°C, pH 7 for 30 minutes (gel only) 20% ethanol |
| Autoclavability | At 121°C, pH 7 for 30 minutes (gel only) |
| Autoclavability | At 121°C, pH 7 for 30 minutes (gel only) |
| Antimicrobial agent | 20% ethanol |
| Autoclavability | At 121°C, pH 7 for 30 minutes (gel only) |
| Antimicrobial agent | 20% ethanol |
| Autoclavability | At 121°C, pH 7 for 30 minutes (gel only) |
| Antimicrobial agent | 20% ethanol |
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Selectivity of the five different prepacked HiPrep Sephacryl™ HR columns.
| TECHNICAL SPECIFICATIONS | |
| Prepacked HiPrep™ Sephacryl HR Columns | |
| Bed dimensions | |
| HiPrep™ 16/60 HiPrep™ 26/60 | 16 × 600 mm 26 × 600 mm |
| Bed volume | |
| HiPrep™ 16/60 HiPrep™ 26/60 | 120 ml 320 ml |
| Sample volume | |
| HiPrep™ 16/60 HiPrep™ 26/60 | < 5 ml < 13 ml |
| Rec. linear flow rate | 5-15 cm/h |
| Max. linear flow rate | 30 cm/h |
| (H2O at 25°C) | |
| Max. pressure over the packed bed during operation | 1.5 bar (22 psi, 0.15 MPa) |
| Column hardware pressure limit | 5 bar (73 psi, 0.5 MPa) |
| Theoretical plates | 5000 m-1 |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |