17-0404-01	HITRAP PROTEIN G HP, 5 X 1 ML
17-0404-03	HITRAP PROTEIN G HP, 2 X 1 ML
17-0405-01	HITRAP PROTEIN G HP,1 X 5 ML
17-0405-03	HITRAP PROTEIN G HP, 5 X 5 ML
17-0406-01	HITRAP HEPARIN HP, 5 X 1 ML
17-0407-01	HITRAP HEPARIN HP,1 X 5 ML
17-0407-03	HiTrap Heparin HP 5x5ml
17-0408-01	HITRAP CHELATING HP, 5 X 1 ML
17-0409-01	HITRAP CHELATING HP, 1 X 5 ML
17-0409-03	HITRAP CHELATING HP 5 X 5 ML
17-0409-05	HITRAP CHELATING HP 100 X 5 ML
17-0412-01	HITRAP BLUE HP, 5 X 1 ML
17-0413-01	HITRAP BLUE HP, 1 X 5 ML
17-0716-01	HITRAP NHS-ACTIV HP, 5 X 1ML
17-0716-30	HiTrap NHS-activated HP, 180 x
17-0717-01	HITRAP NHS-ACTIV HP, 1 X 5 ML
17-0920-03	HITRAP IMAC HP, 5 X 1 ML
17-0920-05	HITRAP IMAC HP, 5 X 5 ML
17-0921-02	HITRAP IMAC FF, 5 X 1ML
17-0921-04	HITRAP IMAC FF, 5 X 5 ML
17-0978-13	HITRAP BUTYL-S FF, 5 X 1ML
17-0978-14	HITRAP BUTYL-S FF, 5 X 5ML

HiTrap™ Protein G HP Columns

• Prepacked with Protein G Sepharose™ High Performance for purification of IgG.
• Designed for use with a syringe, peristaltic pump, or chromatography system.
• Binds 25 mg human IgG/ml medium.
• High capacity and fast binding kinetics.
• Stable from pH 3-9 for use and 2-9 for cleaning

HiTrap™ Protein G HP Columns

Technical Information

TECHNICAL SPECIFICATIONS
Ligand	Recombinant protein G lacking albumin-binding region
Ligand coupling method	N-hydroxysuccinimide activation
Ligand density	» 2 mg protein G/ml medium
Matrix	Highly cross-linked agarose, 6%
Binding capacity	> 25 mg human IgG/ml medium
Average particle size	34 μm
pH stability	3-9 (long term), 2*-9 (short term)
Storage	20% ethanol
Storage temperature	4°C to 8°C
* pH below 3 is sometimes required to elute strongly bound IgG species. However,  protein ligands may hydrolyze at very low pH.

 

Purification of monoclonal rat IgG with HiTrap™ Protein G HP.