英文名称： HiPrep Q XL 16/10
| 28-9365-38 | HiPrep Q XL 16/10 |
| 28-9365-40 | HiPrep SP XL 16/10 |
| 28-9365-41 | HiPrep DEAE FF 16/10 |
| 28-9365-42 | HiPrep CM FF 16/10 |
| 28-9365-43 | HiPrep Q FF 16/10 |
| 28-9365-44 | HiPrep SP FF 16/10 |
| 28-9365-45 | HiPrep Phenyl FF (high sub) 16 |
| 28-9365-46 | HiPrep Phenyl FF (low sub) 16/ |
| 28-9365-47 | HiPrep Butyl FF 16/10 |
| 28-9365-48 | HiPrep Octyl FF 16/10 |
| 28-9365-49 | HiPrep Heparin FF 16/10 |
| 28-9365-50 | GSTPrep FF 16/10 |
| 28-9365-51 | HisPrep FF 16/10 |
| 28-9365-52 | HiPrep IMAC FF 16/10 |
HiPrep™ IEX XL Columns and IEX Sepharose™ XL Lab Packs
- Prepacked with Q Sepharose™ XL* or SP Sepharose XL media, 20-ml preparative anion- and cation-exchange columns provide high loading capacity and fast purifications.
- Optimized for reliable, reproducible separations.
- Q Sepharose XL virus licensed is specifically intended for the purification of viruses.
- Compatible with simple pump-based configurations and other chromatography systems such as ÄKTA™design.
HiPrep™ IEX XL Columns and IEX Sepharose™ XL Lab Packs
Technical Information
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HiPrep™ Q XL 16-10 and HiPrep™ SP XL 16/10 combine high binding capacity with high throughput.
* Licensing information
Separating viral particles with "Q Sepharose™ XL" products may require a license under United States patent 6,537,793 B2 and foreign equivalents owned by Gencell SAS. Such a license is not included with the purchase of Q Sepharose™ XL but is included with the purchase of "Q Sepharose™ XL virus licensed" products.
With the purchase of "Q Sepharose XL virus licensed" the customer is granted a free limited license under US patent 6,537,793 B2 and foreign equivalents owned by Gencell SAS to separate viral particles solely through use of the product purchased.
| TECHNICAL SPECIFICATIONS | |
| Ion exchanger type | |
| Q Sepharose™ XL | Quaternary amino strong anion exchanger |
| SP Sepharose™ XL | Sulfopropyl strong cation exchanger |
| Ionic capacity | |
| Q Sepharose™ XL | 0.18-0.26 mmol Cl-/ml |
| SP Sepharose™ XL | 0.18-0.25 mmol H+/ml |
| Dynamic capacity | |
| Q Sepharose™ XL | > 130 mg BSA/ml medium |
| SP Sepharose™ XL | > 160 mg lysozyme/ml medium |
| Matrix | Dextran attached to 6% highly cross-linked spherical agarose |
| Average particle size | 90 μm |
| pH stability | |
| Q Sepharose™ XL | 2-14 (short term), 2-12 (long term) |
| SP Sepharose™ XL | 3-14 (short term), 4-13 (long term) |
| Chemical stability | All commonly used buffers, nonionic detergents, 1 M NaOH, 6 M guanidine hydrochloride |
| Avoid using | |
| Q Sepharose™ XL | Oxidizing agents, anionic detergents, and buffers |
| SP Sepharose™ XL | Oxidizing agents, cationic detergents, and buffers |
| Storage | 20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature | 4°C to 30°C |
| Prepacked HiPrep™ 16/10 Columns | |
| Column volume | 20 ml |
| Column dimensions | 1.6 × 10 cm |
| Recommended flow rate* | 2-10 ml/min (60-300 cm/h) |
| Max. flow rate* | 10 ml/min (300 cm/h) |
| Max. pressure over packed bed during operation* | 1.5 bar (22 psi, 0.15 MPa) |
| Column hardware pressure limit | 5 bar (73 psi, 0.5 MPa) |
| Storage | 20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature | 4°C to 30°C |
| * H2O at 25°C.
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 expressed in <italic>E. coli</italic> on HiPrep<TM/> Q XL 16/10.'))
Partial purification of the recombinant protein deacetoxycephalosporin C synthase (DAOCS) expressed in E. coli on HiPrep™ Q XL 16/10.
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