| 17-1085-01 | HILOAD 16/10 PHENYL SEPHAROSE | 1 × 20 ml | | 17-1086-01 | HILOAD 26/10 PHENYL SEPHAROSE | 1 × 53 ml | Technical Information
| TECHNICAL SPECIFICATIONS | | Medium | Phenyl Sepharose™ High Performance | | Ligand density | 25 μmol/mg medium | | Binding capacity | 20 mg IgG/ml medium 24 mg HSA/ml medium | | Average particle size | 34 μm | | Rec. linear flow rate | 30-150 cm/h | | Max. pressure | 5 bar (73 psi, 0.5 MPa) | | Chemical stability | Stable in common buffers, chaotropic agents, detergents, and polar organic solvents. | | pH stability | 3-12 (working and long term) 2-14 (short term) | | Storage | 20% ethanol | | Storage temperature | 4°C to 30°C | | Prepacked HiLoad™ Columns | | | Bed volume | | | HiLoad™ 16/10 | 20-22 ml | | HiLoad™ 26/10 | 53-58 ml | | Bed dimensions | | | HiLoad™ 16/10 | 1.6 × 10 cm | | HiLoad™ 26/10 | 2.6 × 10 cm | | Efficiency | > 12 000 m-1 | | Rec. flow rate | Up to 150 cm/h | Max. back pressure over the packed bed during operation | 3 bar (42 psi, 0.3 MPa) | | HiLoad™ column hardware pressure limit | 5 bar (73 psi, 0.5 MPa) | | Storage | 20% ethanol | | Storage temperature | 4°C to 30°C | Separation of conformational isomers of a2-macroglobulin on Phenyl Sepharose™ High Performance
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