17-5079-01	HITRAP RPROTEIN A FF, 5X1ML
17-5079-02	HITRAP RPROTEIN A FF, 2 X 1 ML
17-5080-01	HITRAP RPROTEIN A FF, 1X5ML
17-5080-02	HITRAP RPROTEIN A FF, 5 X 5 ML
17-5110-01	HITRAP IGM PURIFICATION 5X1ML
17-5111-01	HITRAP IGY PURIFICATION 1X5 ML
17-5112-01	HITRAP STREPTAVIDIN HP5X1 ML
17-5130-01	GSTRAP FF, 5X1ML
17-5130-02	GSTRAP FF, 2X1ML
17-5130-05	GSTRAP FF, 100 X 1 ML
17-5131-01	GSTRAP FF, 1X5ML
17-5131-02	GSTRAP FF 5 X 5 ML
17-5131-05	GSTRAP FF, 100 X 5 ML
17-5143-01	HITRAP BENZAMIDINE FF 5 X 1ML
17-5143-02	HITRAP BENZAMIDINE FF 2 X 1 ML
17-5144-01	HITRAP BENZAMIDINE FF 1 X 5 ML
17-5154-01	HITRAP DEAE FF 5 X 5 ML
17-5155-01	HITRAP CM FF, 5 X 5 ML
17-5156-01	HITRAP Q FF, 5 X 5 ML
17-5157-01	HITRAP SP FF, 5 X 5 ML
17-5158-01	HITRAP Q XL, 5 X 1 ML
17-5159-01	HITRAP Q XL, 5 X 5 ML
17-5160-01	HITRAP SP XL, 5 X 1 ML
17-5161-01	HITRAP SP XL, 5 X 5 ML
17-5162-01	HITRAP ANX FF (HIGHSUB)5 X 1ML
17-5163-01	HITRAP ANX FF(HIGHSUB) 5 X 5ML
17-5193-01	HITRAP PHENYL FF (HS), 5 X 5ML
17-5194-01	HITRAP PHENYL FF (LS), 5X 5ML
17-5195-01	HITRAP PHENYL HP, 5 X 5 ML
17-5196-01	HITRAP OCTYL FF, 5 X 5 ML
17-5197-01	HITRAP BUTYL FF, 5 X 5 ML
17-5247-01	HISTRAP HP 5 X 1 ML
17-5247-05	HISTRAP HP 100 X 1 ML
17-5248-01	HISTRAP HP 1 X 5 ML
17-5248-02	HISTRAP HP 5 X 5 ML
17-5248-05	HISTRAP HP 100 X 5 ML
17-5255-01	HISTRAP FF 5 X 5 ML
17-5255-02	HISTRAP FF 100 X 5 ML
17-5281-01	GSTRAP HP 5 X 1 ML
17-5281-05	GSTRAP HP 100 X 1 ML
17-5282-01	GSTRAP HP 1 X 5 ML
17-5282-02	GSTRAP HP 5 X 5 ML
17-5282-05	GSTRAP HP, 100 X 5 ML
17-5286-01	HISTRAP FF CRUDE 5X5 ML
17-5286-02	HISTRAP FF CRUDE 100X5 ML
17-5319-01	HISTRAP FF 5 X 1 ML
17-5319-02	HISTRAP FF 100 X 1 ML
17-5441-22	HITRAP CAPTO S, 5X1 ML
17-5441-23	HITRAP CAPTO S, 5X5 ML
17-5468-51	HiTrap Capto SP ImpRes 5x1mL
17-5468-55	HiTrap Capto SP ImpRes 5x5mL
17-5470-51	HiTrap Capto Q ImpRes 5x1mL
17-5470-55	HiTrap Capto Q ImpRes 5x5mL
17-6002-33	HITRAP IEX SELECTION KIT
18-1142-75	REC PROTEIN PURIF HANDBOOK
28-4010-81	HITRAP_HIPREP 1_16 CONNEC AKTA
28-4014-77	HISTRAP FF CRUDE KIT
28-4017-45	GSTRAP 4B, 5 X 1 ML
28-4017-46	GSTRAP 4B, 100 X 1 ML
28-4017-47	GSTRAP 4B, 1 X 5 ML
28-4017-48	GSTRAP 4B, 5 X 5 ML
28-4017-49	GSTRAP 4B, 100 X 5 ML
28-4058-44	HiTrap Capto Adhere 5x1ml
28-4058-46	5x5 ml Hitrap Capto adhere
28-4082-53	HITRAP MABSELECT, 5 X 1 ML
28-4082-55	HITRAP MABSELECT, 1 X 5 ML
28-4082-56	HITRAP MABSELECT, 5 X 5 ML
28-4082-58	HITRAP MABSELECT XTRA, 5X1ML
28-4082-60	HITRAP MABSELECT XTRA, 1X5ML
28-4082-61	HITRAP MABSELECT XTRA, 5X5ML

HiTrap™ rProtein A FF Columns

• Quick, convenient preparative purification of monoclonal antibodies.
• Packed with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources.
• Recombinant Protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification

HiTrap™ rProtein A FF Columns

Technical Information

The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose™ Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

 

HiTrap™ rProtein A 1 ml and 5 ml columns are packed with 1 ml and 5 ml of rProtein A Sepharose™ Fast Flow, respectively.

 

TECHNICAL SPECIFICATIONS
Column dimensions (i.d.x.h)	0.7 x 2.5 cm (1 ml)
	1.6 x 2.5 cm (5 ml)
Ligand	Recombinant protein A (E. coli)
Ligand coupling method	Epoxy activation, thioether coupling
Ligand density	» 6 mg rProtein A/ml medium
Matrix	Highly cross-linked agarose, 4%
Average particle size	90 μm
Binding capacity	» 50 mg human IgG/ml medium
Dynamic binding capacities †	23 mg mouse monoclonal IgG2a per ml gel
	12 mg mouse monoclonal IgG1 per ml gel
	11 mg monoclonal humanised IgG4 per ml gel
Maximum back pressure	3 bar, 0.3 MPa, 44 psi
Maximum flow rate ‡	4 ml/min (624 cm/h) for 1 ml column
	20 ml/min (600 cm/h) for 5 ml column
Recommended working flow rate	1 ml/min (156 cm/h) for 1 ml column
	5 ml/min (150 cm/h) for 5 ml column
Chemical stability	All commonly used buffers
pH stability	3-10 (working), 2*-11 (short term)
Recommended flow rate	1 ml/ min (1-ml column) 5 ml/min (5-ml column)
Maximum flow rate	4 ml/min (1-ml column) 20 ml/min (5-ml column)
Temperature stability
Regular use	+4-40°C
Storage	+4-8°C
Storage buffer	20% ethanol
Storage	20% ethanol
Storage temperature	4°C to 8°C
* pH below 3 is sometimes required to elute strongly bound IgG species. However,  protein ligands may hydrolyze at very low pH.
† Capacity of HiTrap™ rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.
‡ Room temperature, aqueous buffers
Column: HiTrap™ rProtein A 1 ml. Flow rate: 1 ml/min.
Sample: cell culture supernatants.

 

SDS-PAGE on PhastSystem™ using PhastGel™ Gradient 10-15, silver stained

 

Lane 1:   Low Molecular Weight Calibration Kit (LMW)
Lane 2:   Starting material, diluted 10×
Lane 3:   Flowthrough pool
Lane 4:   Eluted IgG_2b pool

 

Purification of mouse IgG_2b from ascites on HiTrap™ rProtein A FF using a syringe.

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