HiScreen™ Capto™ IEX ImpRes

• Prepacked ready to use columns with different Capto IEX ImpRes media for convenient process development optimization.
• Excellent for method optimization and parameter screening due to the 10 cm bed height.
• Easily connected in series to achieve 20 cm bed height.
• Small bed volume for fast results and minimal sample/buffer consumption.
• Reproducible results, scalable to BioProcess™ columns packed with the same media using the same linear fluid velocity.

HiScreen™ Capto™ IEX ImpRes

Technical Information

HiScreen columns prepacked with Capto IEX ImpRes chromatography media enables the efficient design of intermediate and polishing steps in robust purification processes.

HiScreen columns are part of the process development platform available from GE Healthcare. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

HiScreen Capto Q ImpRes and HiScreen Capto SP ImpRes are prepacked with the BioProcess chromatography media, Capto Q ImpRes and Capto SP ImpRes, respectively. Capto SP ImpRes and Capto Q ImpRes are strong cation and strong anion exchangers that improve the productivity of intermediate to late purification and polishing of a wide range of biomolecules. Both media are part of an expanded high resolution platform based on the high flow agarose Capto product line. By combining the high flow characteristics of Capto media with a small particle size (average 40 μm beads), Capto SP ImpRes and Capto Q ImpRes deliver good pressure-flow properties as well as impressive resolution. Their ability to run at higher flow rates and higher bed heights also increases flexibility in process design. Capto Q ImpRes and Capto SP ImpRes are designed for intermediate purification and polishing of a wide range of recombinant proteins.

GE Healthcare's process development platform.

TECHNICAL SPECIFICATIONS
Media	Capto Q ImpRes Capto SP ImpRes
Column volume	4.7 ml
Column dimensions	0.77 x 10 cm
Recommended fluid velocity1	0.8 to 2.4 ml/min (100 to 300 cm/h)
Column hardware pressure limit	8 bar (0.8 MPa, 117 psi)
Maximum pressure over the packed bed during operation(Dp)	4 bar (0.4 MPa, 59 psi)
Binding capacity2	> 55 mg BSA/ml medium (Capto Q ImpRes) > 48 mg b-Lactoglobulin/ml medium (Capto Q ImpRes) > 70 mg lysozyme/ml medium (Capto SP ImpRes) > 95 mg BSA/ml medium (Capto SP ImpRes)
pH stability3
Capto Q ImpRes	2-14 (short term) 2-12 (long term)
Capto SP ImpRes	3-14 (short term) 4-12 (long term)
Chemical stability	All commonly used aqueous buffers, 1 M sodium hydroxide4, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol
Storage
Capto Q ImpRes	4°C to 30°C in 20% ethanol
Capto SP ImpRes	4°C to 30°C in 20% ethanol, 0.2 M sodium acetate

¹ Water at room temperature.
² Dynamic binding capacity at 10% breakthrough measured at a residence time of 4 minutes (150 cm/h) in a Tricorn™ 5/100 column with 10 cm bed height in 50 mM Tris, pH 8.0 (BSA on Capto Q ImpRes), 20 mM sodium phosphate, pH 7.2 (lysozyme), 50 mM sodium acetate, pH 4.75 (BSA on Capto SP ImpRes).
³ Short term pH: pH interval where the medium can be subjected to cleaning or sanitization-in-place without significant change in function. Long term pH: pH interval where the medium can be operated without significant change in function.
⁴ No significant change in ionic capacity and carbon content after 1 week storage in 1 M NaOH at 40°C.
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