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Ligand: Human IgG, Particle size, d50V1: ~90µm, Matrix: Highly cross-linked 6% agarose

Binding Capacity/mL Chromatography Medium: ~3mg Protein A, pH Stability-Operational2: 3–10

pH Stability-CIP3: 3–10, Pressure/Flow Specification: Base Matrix: 250-400cm/h, 100kpa, XK 50/60 column, Bed Height: 25cm

Flow velocity4: < 300cm/h, using XK 50/30 column with 15cm bed height

Storage: 2 to 8ºC, 20% Ethanol, 50mm K₂HPO₄, 1mMmgCl₂pH 7.2 in 20% Ethanol

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Manufacturer: Whatman/GE Healthcare

Manufacturer Part No: 17-0969-01

Chemical stability: Stable to commonly used aqueous buffers
lgG Sepharose 6 Fast Flow uses the rigid Sepharose 6 Fast Flow matrix, covalently coupled with human IgG, to purify protein A-containing proteins.
Designed for rapid, single-step purification of protein A and protein A fusion conjugates.
Binds at least 2mg protein A/mL resin.
Suitable for tandem affinity purification (TAP) of protein complexes.
Successful use in published research
Applications using IgG Sepharose 6 Fast Flow include:
Tandem affinity purification (TAP) to obtain functional proteins from human cells
Tandem affinity purification to study phosphorylation sites on the Rpb1 C-terminal domain of RNA Polymerase II
Isolating protein A-Cvt19 to study the cytoplasm to vacuole targeting (Cvt) pathway
Compatible empty columns
GE Healthcare offers a wide range of Life Sciences solutions for protein purification, including several types of empty columns. lgG Sepharose 6 Fast Flow is compatible with gravity flow, Tricorn, HiScale, and XK column lines.
Convenient prepacked columns
IgG Sepharose Fast Flow resin is available in the following format:
HiTrap IgG Sepharose FF: convenient for easy use with a syringe, peristaltic pump, or chromatography system