Quasar 670 Amidite

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Quasar® 670 is an indocarbocyanine which fluoresces in the red region of the visible spectrum. This compound is a direct replacement for Cy5™. Quasar 670 amidite is used for the 5' labeling of fluorogenic probes used in 5' nuclease assays, Molecular Beacons™, and other detection assays. This amidite does not contain a DMT protecting group and can only be added to the 5' terminus of the oligo or internally on a free hydroxyl. BHQ®-2 will quench the Quasar 670 moiety.

Properties:

Chemical Name:
Quasar 670 Amidite
Formula:
C46H67F6N5O4P2
Molecular Weight:
929.99
Appearance:
blue solid
Absorption Maximum (Lambda Max):
644
Extinction Coefficient at Lambda max:
187000
Extinction Coefficient at 260 nm:
2800
Fluorescence Maximum:
670

Spectral properties measured in PCR buffer as 5'-labeled poly(T) oligo.

Product Usage:

Synthesis Conditions:
Due to the mild cleavage and deprotection conditions, we recommend using fast deprotecting amidites. Prior to dilution ensure that all product is at the bottom of the vial. Dilute to the recommended concentration and mix thoroughly in sealed vial to ensure that all contents are dissolved. For optimal coupling efficiency, we recommend treating the amidite solution with 3-4 Å activated molecular sieves (10% v/v).
Dilution:
100 碌mol/mL
Deprotection Conditions:
Oligonucleotides labeled with Quasar 670 may be deprotected using concentrated NH4OH, although some degradation can occur. Deprotect the Quasar 670 labeled oligo in concentrated NH4OH for 8 hours at 25 °C or for 1 hour at 60 °C. The use of methylamine should be avoided.

Image of cleaved and deprotected structure:

The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
646.77

Storage and Handling:

Shipping Conditions:
Cold
Storage Conditions:
-15 to -30 °C

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Download SDS for Catalog #BNS-5065 (PDF)

Quasar 670 Amidite

Properties:

Chemical Name:

Quasar 670 Amidite

Formula:

C46H67F6N5O4P2

Molecular Weight:

929.99

Appearance:

blue solid

Absorption Maximum (Lambda Max):

644

Extinction Coefficient at Lambda max:

187000

Extinction Coefficient at 260 nm:

2800

Fluorescence Maximum:

670

Spectral properties measured in PCR buffer as 5'-labeled poly(T) oligo.

Product Usage:

Synthesis Conditions:

Due to the mild cleavage and deprotection conditions, we recommend using fast deprotecting amidites. Prior to dilution ensure that all product is at the bottom of the vial. Dilute to the recommended concentration and mix thoroughly in sealed vial to ensure that all contents are dissolved. For optimal coupling efficiency, we recommend treating the amidite solution with 3-4 Å activated molecular sieves (10% v/v).

Dilution:

100 µmol/mL

Deprotection Conditions:

Oligonucleotides labeled with Quasar 670 may be deprotected using concentrated NH4OH, although some degradation can occur. Deprotect the Quasar 670 labeled oligo in concentrated NH4OH for 8 hours at 25 °C or for 1 hour at 60 °C. The use of methylamine should be avoided.

Additional Information:

Quasar 670 is light sensitive material. Prolonged exposure to light may cause photo bleaching. This product is susceptible to oxidation when exposed to air or stored undessicated.

Image of cleaved and deprotected structure:

The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:

646.77

Related Info

Trademarks
Biosearch, the Biosearch Technologies logo, Black Hole Quencher, BHQ, BHQplus, CAL Fluor, Quasar, Pulsar, Stellaris and SuperROX are registered trademarks of Biosearch Technologies, Inc.

Permitted Product Usage
All products offered for sale by Biosearch Technologies, Inc. (BTI) are sold under the condition that they be used for research purposes only and are prohibited from use in diagnostic or other commercial applications unless explicitly authorized by separate written agreement with BTI.

Black Hole Quencher® (BHQ), CAL Fluor®, Quasar® and Pulsar® dyes (referred to collectively as “BTI Dyes”) are sold to the purchaser only for internal R&D use, and are not to be used for clinical or diagnostic purposes. Neither the BTI Dyes nor the compounds synthesized with them are to be re-packaged or re-sold. Separate licenses to the BTI Dyes may be available for applications other than the aforementioned internal R&D. Please inquire via info@biosearchtech.com.

Patents
The Black Hole Quencher dye technology is protected in the United States and other countries by U.S. patents and continuations numbered 7,019,129, 7,019,129B1, 7,109,312B2, 7,582,432, 8,410,255B2 and 8,440,399B2 issued to Biosearch Technologies, Inc. The CAL Fluor technology is covered by U.S. patent number 7,344,701B2. The Quasar technology is covered by U.S. Patent numbers 7,705,150B2, 7,868, 157B2 and 8,436,153B2. The Pulsar technology is covered by U.S. Patent numbers 7,635,762B2 and 8,119,781B2.

Supercolumns are protected by U.S. Patent 6,761,855 B1, "Column for solid phase processing" issued to Biosearch Technologies, Inc. Contact licensing@biosearchtech.com for more information. Supercolumns for use on ABI 3900 or equivalent rotary cartridge style shoulder mount design are also manufactured under license from McLuen Design, U.S. Patents 8,158,085 and 8,404,196.

Stellaris products are sold under license from Rutgers University and may be used under its patent rights for Research Use Only.

Licensing
Information on licensing programs for the commercial and/or diagnostic use of these products is available by contacting licensing@biosearchtech.com.

Please visit our legal webpage on www.biosearchtech.com for full legal disclosure.

Quasar 670 Amidite

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Properties:

Product Usage:

Storage and Handling:

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{{category.Name}}:

Quasar 670 Amidite

Properties:

Product Usage:

Storage and Handling:

Related Info