Select your real-time PCR instrument

Select your dyes

Recommended dyes are highlighted in blue. Dyes in boldface are available from Biosearch Technologies.

Fluorophore	ABS	EM	Quencher

BHQ® Dye FRET Quenching Range

BHQ-1

BHQ-2

BHQ-3

Notes

Spectral Overlay Charts

ERROR: To view the the chart, you must have a modern browser (recent versions of Chrome or Firefox are recommended) and you must have JavaScript enabled!

Recommended dyes are highlighted in blue. Dyes in boldface are available from Biosearch Technologies.

Fluorophore	ABS	EM	Rec. Quencher
FAM	495	520	BHQ-1
TET	521	536	BHQ-1
CAL Fluor® Gold 540	522	544	BHQ-1
JOE	529	555	BHQ-1
VIC	538	554	N/A
HEX	535	556	BHQ-1
CAL Fluor Orange 560	538	559	BHQ-1
Quasar® 570	548	566	BHQ-2
Cy™ 3	549	566	N/A
TAMRA	557	583	BHQ-2
CAL Fluor Red 590	569	591	BHQ-2
ROX	586	610	BHQ-2
CAL Fluor Red 610	590	610	BHQ-2
Texas Red®	597	616	N/A
CAL Fluor Red 635	618	637	BHQ-2
LC Red® 640	619	637	N/A
Pulsar® 650	460	650	BHQ-2
Cy 5	646	669	N/A
Quasar 670	647	670	BHQ-2*, BHQ-3
Quasar 705	690	705	BHQ-2*, BHQ-3