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DMT-Phosphate (Sulfonyldiethanol) CPG allows for the preparation of oligonucleotides containing a 3' Phosphate group using standard synthesis protocols. After removal of the DMT group from the support and coupling of a phosphoramidite, subsequent cleavage from the support yields a 3' phosphate group. The 500 Å pore size is best suited for the synthesis of DNA sequences up to 50-mers in length.
Properties:
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Chemical Name:
DMT-Phosphate-CPG (O-DMT-2,2'-sulfonyldiethanol-Suc-CPG); 500 Å
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Appearance:
white powder
Product Usage:
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Cleavage Conditions:
Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.
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Deprotection Conditions:
When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
79.98
Image of cleaved and deprotected structure:
Storage and Handling:
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Shipping Conditions:
Ambient
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Storage Conditions:
-15 to -30 °C
