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BHQ®-1 T-Linker 500Å CPG is used to add the non-fluorescent quencher (BHQ-1) to the 3' end of an oligonucleotide. BHQ-1 quencher has maximal absorption in the 480 to 580 nm range, which provides excellent quenching of fluorophores emitting in this range, such as FAM, TET, JOE, and HEX. The BHQ-1 T-Linker Arm CPG has a 3' phosphate followed by a glycolate linkage to the CPG support, which allows for rapid cleavage of the oligo from the support and is labile enough for base-sensitive oligonucleotides. The 500 Å CPG is useful for the synthesis of oligos up to 50-mers in length, especially when larger amounts of product are desired, as it allows for greater nucleoside loading amounts than do supports with larger pore sizes.
Properties:
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Appearance:
dark red powder
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Absorption Maximum (Lambda Max):
548
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Extinction Coefficient at Lambda max:
41600
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Extinction Coefficient at 260 nm:
30100
Spectral properties measured in PCR buffer as 3'-labeled poly(T) oligo.
Product Usage:
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Synthesis Conditions:
Follow the instrument manufacturer's protocol when using this product.
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Cleavage Conditions:
When using fast deprotecting amidites use concentrated Ammonia for 20 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 5 min at 25 °C; or t-butylamine/H2O (1:3) for 45 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 20 min at 25 °C.
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Deprotection Conditions:
When using fast deprotecting amidites (eg. C-Ac, G-DMF) deprotect in concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) deprotect in concentrated ammonia for 5 hours at 60 °C. If you are using base sensitive fluorophores (ie. TAMRA) we recommend deprotecting the oligonucleotide in t-butylamine/H2O (1:3) for 6 hours at 60 °C.
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
1098.98
Storage and Handling:
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Shipping Conditions:
Ambient
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Storage Conditions:
-15 to -30 °C
