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5'-DMT-T-Suc-CPG is used for the placement of T on the 3' end of oligonucleotides. The 500 Å CPG is useful for the synthesis of oligos up to 50-mers in length, especially when larger amounts of product are desired, as it allows for greater nucleoside loading amounts than do supports with larger pore sizes.
Properties:
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Chemical Name:
5'-DMT-T-Suc-CPG; 500 Å
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Appearance:
White Powder
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Extinction Coefficient at 260 nm:
8400
Spectral properties measured in water, for the cleaved and deprotected nucleoside.
Product Usage:
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Cleavage Conditions:
Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.
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Deprotection Conditions:
When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
304.19
Image of cleaved and deprotected structure:
Storage and Handling:
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Shipping Conditions:
Ambient
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Storage Conditions:
+2 to +8 °C
