5'-DMT-G(PAC)-Suc-CPG; 500 Å (RNA)

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5'-DMT-G(PAC)-Suc-RNA CPG is used for the placement of G on the 3' end of oligoribonucleotides. This support has a 2’ acetyl protecting group which is easily cleaved during deprotection. The 500 Å CPG is best suited for the synthesis of RNA sequences up to 50-mers in length. The use of this 5’-DMT protected RNA support is identical to a DNA support with the same protection groups.

Properties:

Chemical Name:
5'-DMT-G(PAC)-Suc-CPG; 500 Å (RNA)
Appearance:
White Powder

Product Usage:

Synthesis Conditions:
Follow the instrument manufacturer's protocol when using this product. If using PAC protected supports or reagents we recommend using phenoxyacetic anhydride or t.butyl phenoacetal acid anhydride(FAST deprotecting capping) as the CAP A reagent. Standard acetic anhydride (CAP A) can cause acetylation of the PAC protected primary amines during synthesis.
Cleavage Conditions:
Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites. We do not recommend using AMA to cleave or deprotect sequences that contain uridine. The AMA may degrade the uridine nucleobase.
Deprotection Conditions:
When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
345.2

Storage and Handling:

Shipping Conditions:
Ambient
Storage Conditions:
-15 to -30 °C

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Download SDS for Catalog #BG5-1200R (PDF)

5'-DMT-G(PAC)-Suc-CPG; 500 Å (RNA)

Properties:

Chemical Name:

5'-DMT-G(PAC)-Suc-CPG; 500 Å (RNA)

Appearance:

White Powder

Product Usage:

Synthesis Conditions:

Follow the instrument manufacturer's protocol when using this product. If using PAC protected supports or reagents we recommend using phenoxyacetic anhydride or t.butyl phenoacetal acid anhydride(FAST deprotecting capping) as the CAP A reagent. Standard acetic anhydride (CAP A) can cause acetylation of the PAC protected primary amines during synthesis.

Cleavage Conditions:

Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites. We do not recommend using AMA to cleave or deprotect sequences that contain uridine. The AMA may degrade the uridine nucleobase.

Deprotection Conditions:

When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.

The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:

345.2

Related Info

Trademarks
Biosearch, the Biosearch Technologies logo, Black Hole Quencher, BHQ, BHQplus, CAL Fluor, Quasar, Pulsar, Stellaris and SuperROX are registered trademarks of Biosearch Technologies, Inc.

Permitted Product Usage
All products offered for sale by Biosearch Technologies, Inc. (BTI) are sold under the condition that they be used for research purposes only and are prohibited from use in diagnostic or other commercial applications unless explicitly authorized by separate written agreement with BTI.

Black Hole Quencher® (BHQ), CAL Fluor®, Quasar® and Pulsar® dyes (referred to collectively as “BTI Dyes”) are sold to the purchaser only for internal R&D use, and are not to be used for clinical or diagnostic purposes. Neither the BTI Dyes nor the compounds synthesized with them are to be re-packaged or re-sold. Separate licenses to the BTI Dyes may be available for applications other than the aforementioned internal R&D. Please inquire via info@biosearchtech.com.

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The Black Hole Quencher dye technology is protected in the United States and other countries by U.S. patents and continuations numbered 7,019,129, 7,019,129B1, 7,109,312B2, 7,582,432, 8,410,255B2 and 8,440,399B2 issued to Biosearch Technologies, Inc. The CAL Fluor technology is covered by U.S. patent number 7,344,701B2. The Quasar technology is covered by U.S. Patent numbers 7,705,150B2, 7,868, 157B2 and 8,436,153B2. The Pulsar technology is covered by U.S. Patent numbers 7,635,762B2 and 8,119,781B2.

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Licensing
Information on licensing programs for the commercial and/or diagnostic use of these products is available by contacting licensing@biosearchtech.com.

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5'-DMT-G(PAC)-Suc-CPG; 500 Å (RNA)

Order Now

Properties:

Product Usage:

Storage and Handling:

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5'-DMT-G(PAC)-Suc-CPG; 500 Å (RNA)

Properties:

Product Usage:

Storage and Handling:

Related Info