{{faqs.Name}}
-
{{category.Name}}:
-
Order Now
| Catalog # | Price | Size/Scale | Note | qty | add | |
|---|---|---|---|---|---|---|
| {{item.catalogId}} | {{ item.userPriceBook.unitPrice | currency }} | {{ item.unitPrice | currency }} | {{item.sizeAndScale}} | {{item.note}} |
{{item.message}}
|
|
| CHECKOUT VIEW CART | ||||||
5'-DMT-A(PAC)-Suc-RNA CPG is used for the placement of A on the 3' end of oligoribonucleotides. This support has a 2’ acetyl protecting group which is easily cleaved during deprotection. The 500 Å CPG is best suited for the synthesis of RNA sequences up to 50-mers in length. The use of this 5’-DMT protected RNA support is identical to a DNA support with the same protection groups.
Properties:
-
Appearance:
White Powder
Product Usage:
-
Synthesis Conditions:
If using PAC protected supports or reagents we recommend using phenoxyacetic anhydride or t.butyl phenoacetal acid anhydride(FAST deprotecting capping) as the CAP A reagent. Standard acetic anhydride (CAP A) can cause acetylation of the PAC protected amines during synthesis.
-
Cleavage Conditions:
Use concentrated ammonia for 90 minutes at 25 °C.Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites. We do not recommend using AMA to cleave or deprotect sequences that contain uridine. The AMA may degrade the uridine nucleobase.
-
Deprotection Conditions:
When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
-
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
329.21
Storage and Handling:
-
Shipping Conditions:
Ambient
-
Storage Conditions:
-15 to -30 °C
