2'-O-Methyl-A(Bz)-Suc-CPG; 500 Å (RNA)

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2’-O-Methyl-A(Bz)-Suc-CPG is used for synthesis of oligonucleotides that contain nuclease resistant 2’-O-methyl linkages to prevent degradation. The 2’-O-Methyl-A(Bz)-Suc-CPG requires no special handling or deprotection reagents. The 500 Å CPG is best suited for the synthesis of RNA sequences up to 50-mers in length.

Properties:

Appearance:
white powder

Product Usage:

Synthesis Conditions:
Follow the instrument manufacturer's protocol when using this product.
Cleavage Conditions:
Use concentrated ammonia for 90 minutes at 25°C or for 30 minutes at 60°C. When using fast deprotecting amidites( CAc; GDMF) 1:1 ammonia:methylamine (AMA) for 25 minutes at 25°C may also be used.
Deprotection Conditions:
When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C. This RNA support is compatible with deprotection chemistries used with 2’ Fluoro; 2' modified phosphoramidites and TBDMS protected reagents.

Image of cleaved and deprotected structure:

The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
343.23

Storage and Handling:

Shipping Conditions:
Ambient
Storage Conditions:
+2 to +8 °C

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Download SDS for Catalog #BG5-1000MR (PDF)

2'-O-Methyl-A(Bz)-Suc-CPG; 500 Å (RNA)

Properties:

Appearance:

white powder

Product Usage:

Synthesis Conditions:

Follow the instrument manufacturer's protocol when using this product.

Cleavage Conditions:

Use concentrated ammonia for 90 minutes at 25°C or for 30 minutes at 60°C. When using fast deprotecting amidites( CAc; GDMF) 1:1 ammonia:methylamine (AMA) for 25 minutes at 25°C may also be used.

Deprotection Conditions:

When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C. This RNA support is compatible with deprotection chemistries used with 2’ Fluoro; 2' modified phosphoramidites and TBDMS protected reagents.

Additional Information:

AMA is not recommended for sequences containing uridine bases.

Image of cleaved and deprotected structure:

The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:

343.23

Related Info

Trademarks
Biosearch, the Biosearch Technologies logo, Black Hole Quencher, BHQ, BHQplus, CAL Fluor, Quasar, Pulsar, Stellaris and SuperROX are registered trademarks of Biosearch Technologies, Inc.

Permitted Product Usage
All products offered for sale by Biosearch Technologies, Inc. (BTI) are sold under the condition that they be used for research purposes only and are prohibited from use in diagnostic or other commercial applications unless explicitly authorized by separate written agreement with BTI.

Black Hole Quencher® (BHQ), CAL Fluor®, Quasar® and Pulsar® dyes (referred to collectively as “BTI Dyes”) are sold to the purchaser only for internal R&D use, and are not to be used for clinical or diagnostic purposes. Neither the BTI Dyes nor the compounds synthesized with them are to be re-packaged or re-sold. Separate licenses to the BTI Dyes may be available for applications other than the aforementioned internal R&D. Please inquire via info@biosearchtech.com.

Patents
The Black Hole Quencher dye technology is protected in the United States and other countries by U.S. patents and continuations numbered 7,019,129, 7,019,129B1, 7,109,312B2, 7,582,432, 8,410,255B2 and 8,440,399B2 issued to Biosearch Technologies, Inc. The CAL Fluor technology is covered by U.S. patent number 7,344,701B2. The Quasar technology is covered by U.S. Patent numbers 7,705,150B2, 7,868, 157B2 and 8,436,153B2. The Pulsar technology is covered by U.S. Patent numbers 7,635,762B2 and 8,119,781B2.

Supercolumns are protected by U.S. Patent 6,761,855 B1, "Column for solid phase processing" issued to Biosearch Technologies, Inc. Contact licensing@biosearchtech.com for more information. Supercolumns for use on ABI 3900 or equivalent rotary cartridge style shoulder mount design are also manufactured under license from McLuen Design, U.S. Patents 8,158,085 and 8,404,196.

Stellaris products are sold under license from Rutgers University and may be used under its patent rights for Research Use Only.

Licensing
Information on licensing programs for the commercial and/or diagnostic use of these products is available by contacting licensing@biosearchtech.com.

Please visit our legal webpage on www.biosearchtech.com for full legal disclosure.

2'-O-Methyl-A(Bz)-Suc-CPG; 500 Å (RNA)

Order Now

Properties:

Product Usage:

Storage and Handling:

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2'-O-Methyl-A(Bz)-Suc-CPG; 500 Å (RNA)

Properties:

Product Usage:

Storage and Handling:

Related Info