| 产品详情 |
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| Antigenic Specificity | DRIP130 |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | mouse, human |
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| Isotype | n/a |
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| Format | delipidated & defibrinated |
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| Size | 500 µL |
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| Concentration | 85 mg/mL |
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| Applications | ELISA, Immunoprecipitation, Western Blot. Anti-DRIP-130 detects a 130 kDa band by immunoblot analysis using a dilution of 1:1,000. For immunoprecipitation a dilution of 1:100 or 1:200 is suggested; pre-clearing with a non-specific rabbit IgG is helpful to reduce background. Optimal titers for applications should be determined by the researcher. Rockland Immunochemical's anti-DRIP130 has been used to detect DRIP130 using nuclear extracts from mouse thymus, spleen, and brain. Unfortunately, we have not tested reactivity on any cell line extracts. The following standard procedure is recommended: separate 10 micrograms of thymus nuclear extract by 7.5% SDS-PAGE (0.75 to 1 mm thick). Transfer to nitrocellulose without SDS in the transfer buffer (pre-equilibrate the gel in transfer buffer for 30 min prior to transfer). Blocking is performed with 5% non-fat dry milk in TTBS for 1 hr at room temperature. Incubate anti-DRIP130 antibody at a 1:1,000 for 1 hr at room temperature. Dilute Donkey anti-Rabbit IgG-HRP 1:5,000 and react 1 hr at room temperature. A predominant band at ~130 KDa is detected under these conditions. Control or normal rabbit serum fails to recognize the 130 kDa protein. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This antiserum was processed by delipidation and defibrination and is directed against mouse DRIP130. This reagent can be used to determine the presence of DRIP130 various immunological assays. Cross-reactivity occurs with human DRIP-130. Reactivity with DRIP-130 isolated from other species is unknown. |
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| Immunogen | This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to mouse DRIP-130 residues 897-916 (based on human DRIP-130 residue numbering) crosslinked to KLH. |
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| Other Names | rabbit anti-DRIP130antibody, Mediator of RNA polymerase II transcription subunit 23, Mediator complex subunit 23, Cofactor required for Sp1, transcriptional activation subunit 3, CRSP complex subunit 3, CRSP3, Transcriptional coactivator CRSP130, ARC130, DRIP130, KIAA1216, SUR2 |
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| Gene, Accession # | MED23, DRIP130, Gene ID: 9439, NCBI: 28558074, UniProt: Q9ULK4 |
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| Catalog # | 100-401-239 |
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| Price | |
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| Order / More Info | DRIP130 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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