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| Product Name | Luciferase Reporter BioAssay™ Kit |
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| Description | Firefly luciferase has been used as a sensitive reporter for studying gene regulation and function. Luciferase provides a 1000-fold increase in sensitivity in comparison to the standard chloramphenicol acetyltransferase (CAT) assay. Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction. The light emission from the reaction can be recorded using a luminometer. Sample type: Cell and Tissue culture homogenates (eukaryotic cells) Intended Use: Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells. Test Principle: For measurement of expressed luciferase in vitro, luciferase is first extracted from transfected cells through cell lysis. Then CoA, ATP, Mg2+ and buffer are added to the lysate (Inclusion of CoA yields a nearly constant light emission rather than typical flash Kinetics). The luminescent reaction is then triggered by an inj |
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| Size | 200 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 169702 |
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| Order / More Info | Luciferase Reporter BioAssay™ Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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