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| Product Name | Interleukin 2, Human (IL-2), BioAssay™ ELISpot Kit |
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| Description | Intended Use: This 2.5 hour ELISpot kit is developed to detect and visualize of single cells secreting human IL-2. Principle: Add stimulated cells or cells and stimulant to the wells and incubate at 37°C in CO2 incubator for a specified period. Secreted human IL-2 binds to antibody coated microtiter plate. microtiter plate coated with human IL-2 Capture Antibody. Cells and unbounded proteins are washed away. Streptavidin-AP or Streptavidin-HRP is added and binds to the biotinylated detection antibody. Unbounded proteins are washed away. Biotinylated detection antibody is added and binds to the secreted human IL-2. Substrate Solution is added. A colored precipitate forms and appears as spots at the sites of human IL-2 secreting location. Each individual spot representing an individual human IL-2 secreting cell. Background: Interleukin 2 (IL-2) is a lymphokine synthesized and secreted primarily by T helper lymphocytes that have been activated by stimulation with certain mitogens or b |
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| Size | 96 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | I7663-28K |
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| Order / More Info | Interleukin 2, Human (IL-2), BioAssay™ ELISpot Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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