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Product Name | Interleukin 11, Human (IL-11) BioAssay™ ELISA Kit |
Description | This IL-11 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-11. Standards or samples are then added to the appropriate microtiter plate wells and incubated. After washing to remove unbound IL-11 and other components of the sample, biotin-conjugated polyclonal antibody specific to IL-11 is added and incubated. If present, IL-11 will bind and become immobilized by the antibody pre-coated on the wells and then become ``sandwiched'' by the biotin conjugate. In order to quantitatively determine the amount of IL-11 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' |
Size | 96 tests |
Concentration | n/a |
Applications | ELISA |
Other Names | n/a |
Gene, Accession, CAS # | n/a |
Catalog # | I8433-10B |
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Order / More Info | Interleukin 11, Human (IL-11) BioAssay™ ELISA Kit from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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