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Product Name | hs-cTnI (High Sensitivity Cardiac Troponin I) BioAssay™ ELISA Kit (Canine) |
Description | Sample Type: Serum, plasma, tissue homogenates and other biological fluids. Intended Use: Sandwich ELISA, Double Antibody Sensitivity: <9.375pg/ml Range: 15.625-1000pg/ml Specificity: Canine Test Principle: This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-hs-cTnI antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-hs-cTnI antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the hs-cTnI amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concent |
Size | 48 tests, 96 tests |
Concentration | n/a |
Applications | ELISA |
Other Names | n/a |
Gene, Accession, CAS # | n/a |
Catalog # | 369976 |
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Order / More Info | hs-cTnI (High Sensitivity Cardiac Troponin I) BioAssay™ ELISA Kit (Canine) from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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