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| Antigenic Specificity | VDAC/Porin, Internal |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | mouse, rat, human, bovine, swine, goat |
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| Isotype | n/a |
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| Format | ready to use |
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| Size | 100 µL |
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| Concentration | sufficient to run 10 immunoblot tests |
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| Applications | ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. Ready to Use Anti-VDAC/Porin Antibody has been optimized and validated in western blot at 1:1000 dilution. This Anti-VDAC/Porin (RTU) Antibody is sufficient to run 10 western blots. Expect a band approximately 30-33kDa in size corresponding to VDAC/Porin by western blotting in appropriate cell lysate or extract. Although not tested, this antibody is likely functional in ELISA and Immunohistochemistry. Optimal titers for applications should be determined by the researcher. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | RTU Anti-VDAC/Porin Antibody is directed against human VDAC1/Porin1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial). VDAC/Porin Antibody recognizes VDAC (also known as Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL) which is an outer membrane mitochondrial protein. The VDAC proteins are ~30-33 kDa (some isoforms are larger - see below). The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mito-chondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest. This antibody can be used as a loading control with whole cell lysates and total mitochondrial preparations. All Rockland Immunochemical's VDAC/Porin antibodies are affinity-purified to assure both high affinity and specificity. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays. |
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| Immunogen | VDAC/Porin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 185-197 of Human VDAC1/Porin1. |
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| Other Names | Loading Control Antibody, VDAC-1 Antibody, POR1 Antibody |
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| Gene, Accession # | VDAC1, Gene ID: 7416, UniProt: P21796, NCBI: NP 003365.1 |
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| Catalog # | 600-401-882-RTU |
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| Price | |
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| Order / More Info | VDAC/Porin, Internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Thakurela S, Garding A, Jung RB, Mueller C, Goebbels S, White R, Werner HB, Tiwari VK. (2016) The transcriptome of mouse central nervous system myelin. Sci Rep. 2016 May 13;6:25828. doi: 10.1038/srep25828. Petermann P, Haase I, Knebel-Moersdorf D. (2009) Impact of Rac1 and Cdc42 signaling during early herpes simplex virus type 1 infection of keratinocytes. J Virol. 2009 Oct;83(19):9759-72. doi: 10.1128/JVI.00835-09. Epub 2009 Jul 29. |
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