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| Antigenic Specificity | Myosin phospho S19/phospho S20, N-terminus |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human |
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| Isotype | n/a |
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| Format | immunoaffinity purified |
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| Size | 100 µg |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. Rabbit Anti-Myosin pS19/sP20 Antibody was tested by ELISA, immunohistochemistry and western blotting. Immunoblotting was used to show reactivity with unstimulated and stimulated cardiac myocytes, 3T3 whole cell lysates, and regulatory light chain and smooth muscle phospho recombinant protein. The antibody was also reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional by immunoprecipitation. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The antibody is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phosphospecific polyclonal antibody is specific for the phosphorylated pS19/pS20 form of the protein, depending on the source origin of the protein. Reactivity with non-phosphorylated myosin light chain is less than 1% by ELISA. Cross reactivity is expected with myosin light chain from human and mouse. Reactivity with the protein from other species has not been determined. However, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected. |
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| Immunogen | Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH). |
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| Other Names | rabbit anti-Myosin p19/pS20 antibody, Myosin regulatory light chain 12A, Myosin regulatory light chain MRLC3, Myosin regulatory light chain 2 nonsarcomeric, Myosin RLC, MLC-2B, HEL-S-24, Epididymis secretory protein Li 24, MLCB |
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| Gene, Accession # | MYL12A, Gene ID: 10627, NCBI: AAH16372.1, UniProt: P19105 |
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| Catalog # | 600-401-416 |
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| Price | |
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| Order / More Info | Myosin phospho S19/phospho S20, N-terminus Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Sanchez-Elordi, E., Vicente-Manzanares, M., Diaz, E., Legaz, M. E., & Vicente, C. (2016). Plant-pathogen interactions: Sugarcane glycoproteins induce chemotaxis of smut teliospores by cyclic contraction and relaxation of the cytoskeleton. South African Journal of Botany, 105, 66-78.Jessica R. Edelson, David L. Brautigan. (2011) The Discodermia calyx Toxin Calyculin A Enhances Cyclin D1 Phosphorylation and Degradation, and Arrests Cell Cycle Progression in Human Breast Cancer Cells. Toxins 2011, 3(1), 105-119; doi:10.3390/toxins3010105. Logue JS, Cartagena-Rivera AX, Baird MA, Davidson MW, Chadwick RS, Waterman CM. (2015) Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration. Elife. 2015 Jul 11;4:e08314. doi: 10.7554/eLife.08314. |
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