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Antigenic Specificity | Myosin, N-terminus |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, mouse, rat |
Isotype | n/a |
Format | immunoaffinity purified |
Size | 100 µg |
Concentration | 0.98mg/mL |
Applications | ELISA, Immunoprecipitation, Western Blot. This affinity-purified antibody was tested by ELISA and immunoblotting and was found to be reactive with both the unphosphorylated and mono-phosphorylated forms of the protein. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Cross reactivity is expected with myosin light chain from human, mouse and rat sources. Reactivity with the protein from other species has not been determined; however, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected. |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 12-27 of human myosin light chain protein. |
Other Names | rabbit anti-Myosin antibody, Myosin regulatory light polypeptide 9, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, Myosin regulatory light chain 2, smooth muscle isoform, Myosin RLC, MLC-2C, 20 kDa myosin light chain |
Gene, Accession # | MY19, Gene ID: 98932, NCBI: 38605043, UniProt: P24844 |
Catalog # | 600-401-938 |
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Order / More Info | Myosin, N-terminus Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | Logue JS, Cartagena-Rivera AX, Baird MA, Davidson MW, Chadwick RS, Waterman CM. (2015) Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration. Elife. 2015 Jul 11;4:e08314. doi: 10.7554/eLife.08314. |
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