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| Antigenic Specificity | Maltose Binding Protein (MBP) Epitope Tag |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | n/a |
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| Isotype | n/a |
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| Format | purified |
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| Size | 1 mg |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, Western Blot. Anti-MBP is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. Anti-MBP is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli. By Western blot, a band is seen at ~ 42 kDa representing MBP. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This IgG purified antibody is directed against MBP and is useful in determining its presence in various assays. This polyclonal anti-MBP tag antibody detects over-expressed proteins containing the MBP epitope tag. To date this antibody has reacted with all MBP tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins. |
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| Immunogen | This antibody was purified from whole rabbit serum prepared by repeated immunizations with the MBP epitope tag recombinant protein. |
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| Other Names | rabbit anti-MBP Epitope Tag Antibody, rabbit anti-Maltose Binding Protein Antibody |
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| Gene, Accession # | n/a |
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| Catalog # | 200-401-385 |
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| Price | |
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| Order / More Info | Maltose Binding Protein (MBP) Epitope Tag Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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