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Antigenic Specificity | Angiopoietin 1, N-terminus |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, mouse |
Isotype | n/a |
Format | unconjugated |
Size | 200 µL |
Concentration | 80 mg/mL |
Applications | ELISA, Immunohistochemistry, Western Blot. Suitable for western blotting, Immunohistochemistry (IHC) and other antibody based assays. A 1:500 dilution is recommended for western blotting. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean and strong signal. Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Antiopoietin 1 is directed against mouse angiopoietin1 and shows no reactivity with mouse angiopoietin2. A 57 kDa band is detected when assayed by immunoblot. This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2. |
Immunogen | This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide N-Q-R-R-N-P-E-N-G-G-R-R-Y-N-R-I-Q-H-G-Q corresponding to a region (aa 21-40) near the N-terminus of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. |
Other Names | rabbit anti-Angiopoietin 1 Antibody, rabbit anti-ANG1 antibody, AGP 1 antibody, AGP1 antibody, AGPT antibody, ANG 1 antibody, ANG1 antibody, Angiopoietin1 antibody, ANGPT 1 antibody, ANGPT1 antibody, KIAA0003 antibody |
Gene, Accession # | ANG1, Gene ID: 11600, NCBI: 46048213, UniProt: O08538 |
Catalog # | 100-401-403 |
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Order / More Info | Angiopoietin 1, N-terminus Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
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