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| Antigenic Specificity | Histone H2AvD pS137, internal |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | Drosophila melanogaster |
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| Isotype | n/a |
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| Format | immunoaffinity purified |
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| Size | 100 µg |
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| Concentration | 1.0 mg/ml |
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| Applications | ELISA, Immunohistochemistry, Western Blot. Histone H2AvD pS137 Antibody has been tested for use in ELISA, Immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14 kDa in size corresponding to phosphorylated H2AvD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract. Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS137 of H2AvD protein. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Affinity purified Anti-Histone H2AvD pS137 Antibody is directed against the phosphorylated form of Drosophila H2AvD protein at the pS137 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against Drosophila H2AvD pS137 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA. A BLAST analysis was used to suggest little to no cross reactivity with H2AvD proteins from other sources based on a comparison using the immunizing sequence. Reactivity against homologues from other sources is not known. |
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| Immunogen | Histone H2AvD pS137 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 132-141 of Drosophila melanogaster (fruit fly) H2AvD protein. |
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| Other Names | rabbit anti-H2AvD pS137 antibody, rabbit anti-Histone H2A.v pS137 antibody, H2AvD protein antibody, H2A.F/Z, H2A.Z, H2AvD, His2AvD, His2Av |
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| Gene, Accession # | HIS2AV, Gene ID: 43229, NCBI: 17738227, UniProt: P08985 |
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| Catalog # | 600-401-914 |
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| Price | |
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| Order / More Info | Histone H2AvD pS137, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Bosveld, F., Rana, A., van der Wouden, P. E., Lemstra, W., Ritsema, M., Kampinga, H. H., & Sibon, O. C. (2008). De novo CoA biosynthesis is required to maintain DNA integrity during development of the Drosophila nervous system. Human molecular genetics, 17(13), 2058-2069.Ryu T, Spatola B, Delabaere L, Bowlin K, Hopp H, Kunitake R, Karpen GH, Chiolo I. (2015) Heterochromatic breaks move to the nuclear periphery to continue recombinational repair. Nat Cell Biol. 2015 Nov;17(11):1401-11. doi: 10.1038/ncb3258. Epub 2015 Oct 26.Vujatovic O, Zaragoza K, Vaquero A, Reina O, Bernues J, Azorin F. (2012) Drosophila melanogaster linker histone dH1 is required for transposon silencing and to preserve genome integrity. Nucleic Acids Res. 2012 Jul;40(12):5402-14. doi: 10.1093/nar/gks224. Epub 2012 Mar 9. |
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